苍附导痰汤对多囊卵巢综合征大鼠oatp4a1、代谢紊乱与肠道菌群的影响

目的]探究苍附导痰汤对多囊卵巢综合征大鼠oatp4a1、代谢紊乱与肠道菌群的影响。方法]选取40只SPF级SD雌性大鼠，随机分为正常（N）组、模型（M）组、低剂量苍附导痰汤（D）组、高剂量苍附导痰汤（H）组，每组10只，对M组、D组、H组采用脱氢表雄酮（DHEA）及高脂饲料建立多囊卵巢综合征模型，N组不建立该模型，建模后对D组灌胃1.42 g/kg苍附导痰汤，对H组灌胃5.68 g/kg苍附导痰汤，N组、M组同期给予同体积生理盐水灌胃，苏木精-伊红（HE）染色法检测卵巢组织病理形态，免疫组化法检测卵巢组织中有机阴离子转运多肽4a1(oatp4a1)蛋白表达，血糖仪、酶联免疫吸附测定（ELISA）法及放射免疫法检测大鼠血清中代谢相关水平，肠道菌群选择性培养基检测大鼠肠道菌群数量变化。结果]与N组比较，M组大鼠动情前期、卵巢指数明显升高（P<0.05），动情期明显降低（P<0.05）；与M组比较，D组、H组大鼠动情前期、卵巢指数显著降低（P<0.05），动情期显著升高（P<0.05），且H组比D组变化明显（P<0.05）。N组大鼠卵巢组织及卵泡生长正常，...

Effects of Cangfu Daotan Decoction on OATP4A1, metabolic disorders and intestinal flora in rats with polycystic ovary syndrome

Objective] To explore the effects of Cangfu Daotan Decoction on oatp4a1, metabolic disorders and intestinal flora in rats with polycystic ovary syndrome. Methods] The 40 SPF SD female rats were selected and randomly divided into normal group(N), model group(M), low dose pale attached guide phlegm decoction(D) group, high dose pale attached guide phlegm decoction(H) group, each group of 10, only the M group, D and H group adopts the use of DHEA(DHEA) and high fat feed polycystic ovary syndrome model was established. The N group was not set up the model. After modeling, D group was given 1.42 g/kg Cangfu Daotan Decoction;H group was given 5.68 g/kg Cangfu Daotan Decoction;N group and M group were given the same volume of normal saline;HE staining method was used to detect the pathological morphology of ovarian tissue, immunohistochemistry method was used to detect oatp4a1 protein expression in ovarian tissue. Serum metabolism-related levels of rats were detected by glucose analyzer, ELISA and radioimmunoassay, and the number of intestinal flora was detected by selective medium. Results] Compared with N group, M group rats pro oestrus and ovarian index increased significantly(P<0.05), estrus significantly decreased(P<0.05), compared with M group, the D and H group rats pro oestrus and ovarian index decreased significantly(P<0.05), a significant rise in estrus(P<0.05), and than changes in D group and H group was obviously(P<0.05). N group of normal ovarian tissue of rats and follicular growth, visible multiple corpus luteum follicle and different development period, and interstitial, shape good, normal stroma in deep dyeing of mast cells, M group visible ovarian cortex of rats with polycystic change, corpus luteum number decreased significantly, visible more atresia follicles, follicular cell necrosis, thickening, more visible and obvious inflammatory cell infiltration. The pathological changes of ovary in D group and group H group were significantly improved, and the number of luteum increased gradually. Compared with N group, oatp4a1 protein expression in ovary tissues of rats in M group was significantly decreased(P<0.05), oatp4a1 protein expression in ovary tissues of rats in D group and H group was significantly increased compared with M group(P<0.05), and H group was significantly higher than D group(P<0.05). Compared with N group, serum FBG, FINS and HOMA-IR in M group were significantly increased(P<0.05), and SERUM APN in M group was significantly decreased(P<0.05). Compared with M group, serum FBG, FINS and HOMA-IR in D group and H group were significantly decreased(P<0.05), and serum APN was significantly increased(P<0.05). The change of H group was significantly higher than that of D group(P<0.05). Compared with N group, M group enterobacter number of rats significantly increased(P<0.05), enterococcus and lactobacillus, bifidobacterium quantity decreased obviously(P<0.05). Compared with M group, D and H group in the rat intestinal bacteria number decreased significantly(P<0.05), enterococcus and lactobacillus, bifidobacterium quantity increased significantly(P<0.05), and the change of H group was significantly higher than that of D group(P<0.05). Conclusion] Cangfu Daotan Decoction can significantly promote oatp4a1 protein expression in rats with polycystic ovary syndrome, improve metabolic disorders and intestinal flora of rats.