| 人牙周膜成纤维细胞与牙龈成纤维细胞差异表达一条新基因的克隆 |
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| 引用本文： | 郭希民,吴补领,肖明振,蒲勤,赵忠良. 人牙周膜成纤维细胞与牙龈成纤维细胞差异表达一条新基因的克隆[J]. 牙体牙髓牙周病学杂志, 2000, 10(6): 343-346 |
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| 作者姓名： | 郭希民 吴补领 肖明振 蒲勤 赵忠良 |
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| 作者单位： | 1. 第四军医大学：口腔医学院
2. 基础部，陕西西安，710032 |
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| 基金项目： | 国家自然科学基金资助课题(39670783)高等学校骨干教师资助计划资助 |
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| 摘 要： | 目的：克隆人牙周膜成纤维细胞（ｐｅｒｉｏｄｏｎｔａｌ ｌｉｇａｍｅｎｔ ｆｉｂｒｏｂｌａｓｔ，ＰＤＬＦ）与牙龈成纤维细胞（ｇｉｎｇｉｖａｌ ｆｉｂｒｏｂｌａｓｔ，ＧＦ）差异表达的新基因。方法：采用基于ＰＣＲ和消减杂交的基因克隆技术构建体外培养的人ＰＤＬＦ与ＧＦ差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的的克隆，并进行测序和计算机分析。结果：从构建的人ＰＤＬＦ与ＧＦ细胞差异表达基因的扣除
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| 关 键 词： | 牙周膜 成纤维细胞 牙龈 PCR 消减杂交 基因克隆 |
| 文章编号： | l005-2593(2000)06-0343-04 |
| 修稿时间： | 2000-09-13 |
Cloning of a new gene expressed differently in human periodontal ligament fibroblast in comparison with gingival fibroblasts |
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| Abstract: | AIM: To clone a new gene that is differentially expressed in human PDLF in comparison with GF.METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot. Desired genes were sequenced and then analyzed by computer. RESULTS: We cloned a 818bp new gene from a PDLF cDNA subtractive library. CONCLUSION: We cloned a new gene from the subtuctive htnan PDLF cDNA library. |
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| Keywords: | PCR PDLF GF PCR subtractive hybridization gene cloning |
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