谷胱苷肽S转移酶M1和T1基因多态性与喉及下咽癌易感性的关系

目的：研究谷胱苷肽S转移酶M1（GSTM1）、谷胱苷肽S转移酶T1（GSTT1）基因多态性与喉及下咽癌易感性的关系。方法：采用多重PCR技术,对76例喉、下咽癌患者（患者组）和与之匹配的76例对照组进行GSTM1、GSTT1基因型检测。结果：患者组与对照组GSTM1基因缺失频率分别为59.2%和42.1%,差异有统计学意义（P〈0.01）,GSTM1基因缺失与喉、下咽癌易感性有关（OR=1.935,95%CI=1.069～3.510）;GSTT1基因缺失频率分别为57.9%和51.3%,差异无统计学意义。联合分析未发现两种基因在喉、下咽癌发生中具有协同作用。GSTM1基因缺失同时暴露于吸烟者患喉、下咽癌的风险性显著增加（OR=5.545,95%CI=2.158～13.528）。结论：GSTM1基因缺失可能增加个体患喉、下咽癌的易感性,且与吸烟在喉、下咽癌的发生中具有协同作用。GSTT1基因型可能与本市区喉、下咽癌发生无关。

Polymorphisms of GSTM1,GSTT1 and susceptibility of laryngeal and hypopharyngeal carcinomas

Objective:To study the relationship between genetic polymorphisms of GSTM1 GSTT1 and the susceptibility of laryngeal and hypopharyngeal carcinomas(LHC).Method:The GSTM1 an GSTT1 genotypes were determined by multiplex PCR analysis in 76 LHC patients and 76 population controls.The association be tween the genotypes and LHC risk was measured by odds ratios(ORs)and 95% confidence intervals(95%Cls).Resuit:The frequency of GSTM1 null genotype was 59.2% in the LHC patients and 42.1% in controls(OR=1.935,95%CI=1.069-3.510),the difference was significant(P＜0.01).The frequency of GSTT1 null genotype was 57.9% in the LHC patients and 51.3% in controls.The difference was not significant(P＞0.05).In smokers,the risk of the LHC increased in subjects of GSTM1 null genotype(OR=5.545,95%CI=2.158-13.528).Conclusion:GSTM1 polymorphisms are associated with susceptibility to the LHC.It has the synergistic effects with smoking in the development of the LHC.GSTT1 genotypes might have no association with risk of the LHC in urban Linyi.