| 当归的指纹图谱和Q-TOF-MS质谱联用研究 |
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| 引用本文： | 罗丹,刘智华,潘纯国,朱根华.当归的指纹图谱和Q-TOF-MS质谱联用研究[J].江西中医学院学报,2012,24(1):34-36. |
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| 作者姓名： | 罗丹 刘智华 潘纯国 朱根华 |
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| 作者单位： | 1. 江西中医学院现代中药制剂教育部重点实验室,南昌,330004
2. 江西省肿瘤医院,南昌,330029 |
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| 基金项目： | 学位与研究生教育教学改革研究项目，江西省科技计划项目 |
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| 摘 要： | 目的：用高效液相色谱法建立当归药材的指纹图谱,为其品质控制提供依据。方法：采用Phenomenex luna C18色谱柱（4.6 mm×250 mm,5μm）;乙腈-0.5%醋酸水溶液为流动相,梯度洗脱,检测波长为270 nm,流速1 ml/min。结果：建立了当归药材的HPLC指纹图谱。HPLC-MS鉴定出阿魏酸,洋川芎内酯,川芎内酯A,丁基苯酞,E-藁本内酯,Z-藁本内酯,E-丁烯基酞内酯和Z-丁烯基苯酞共8个化学成分。结论：采用该法可为控制当归药材的内在质量提供依据。
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| 关 键 词： | 当归 液质联用 指纹图谱 |
High performance liquid chromatography -mass spectrometry analysis of Radix Angelica Sciensis |
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| LUO Dan
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LIU Zhi-hua
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PAN Chun-guo
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ZHU Gen-hua.High performance liquid chromatography -mass spectrometry analysis of Radix Angelica Sciensis[J].Journal of Jiangxi College of Traditional Chinese Medicine,2012,24(1):34-36. |
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| Authors: | LUO Dan LIU Zhi-hua PAN Chun-guo ZHU Gen-hua |
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| Institution: | 1.Key Laboratory of Modern Preparation of TCM,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nangchang 330004; 2.Jiangxi Cancer hospital,Nangchang 330029 |
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| Abstract: | Objective:To analyze the chemical components in Danggui(the roots o f Angelica sinensis(Oliv.) D iel).Methods:HPLC-MS /MS was used to identify the main components in Danggu.The mobile phase of HPLC consisted of 0.5 % acetic acid in water and MeOH,analytical column was Phenomenex(250 mm×4.6 mm,5um),flow rate 1.0 mL/min,injected volume 10uL.Results:The ionization source was ESI in positive ion mode.Results Seven compounds in Danggui were tentatively identified via HPLC-MS /MS.Conclusion:It provides an accurate and fast method to control the quality of Danggui. |
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| Keywords: | Danggui HPLC-MS /MS Fingerprint |
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