A novel hepatitis B virus mutant coexisting with wild type virus in a carrier with negative HBsAg yet positive HBeAg and anti-HBs |
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| Institution: | 1. Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China;2. Department of Hepatobiliary Surgery, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China;3. Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing University Medical School, 321 Zhong Shan Road, Nanjing 210008, China;4. Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, China;1. Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon, South Korea;2. Well Aging Research Center, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea;3. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea;4. Department of Molecular Medicine, Chonnam National University Medical School, Gwangju, South Korea;5. The Future Life & Society Research Center, Chonnam National University, Gwangju, South Korea;1. Centre national de transfusion sanguine (CNTS), Libreville, Gabon;2. Laboratoire national de santé publique, Libreville, Gabon;1. Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Tokyo, Japan;2. NCGM-BMH Medical Collaboration Center, Hanoi, Viet Nam;3. Hanoi Department of Health, Hanoi, Viet Nam;4. Hanoi Lung Hospital, Hanoi, Viet Nam;5. Department of Microbiology, National Lung Hospital, Hanoi, Viet Nam;6. Department of Microbiology, Hanoi Lung Hospital, Hanoi, Viet Nam;7. National Center for Global Health and Medicine, Tokyo, Japan;8. Department of Pathophysiology and Host Defense, Research Institute of Tuberculosis JATA, Tokyo, Japan;9. Bureau of International Medical Cooperation, National Center for Global Health and Medicine, Tokyo, Japan;1. Department of Biotechnology, College of Life Science & Biotechnology, Yonsei University, Seoul, South Korea;2. Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, Seoul, South Korea |
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| Abstract: | BackgroundOccult infection of hepatitis B virus (HBV) has important impacts on both public health and clinical medicine.ObjectivesTo characterize the sequences of HBV S region in a chronic carrier with occult HBV infection.Study designSerological markers for HBV were tested by commercial kits. Western blotting was performed to detect HBsAg. PCR was used to amplify HBV S region; the resultant products were sequenced directly and cloned and then sequenced.ResultsTests with commercial kits showed that the carrier was HBsAg negative yet HBeAg positive. HBsAg was positive in Western blotting analysis. Although anti-HBs titers were as high as 5356–11,578 mIU/ml, serum HBV DNA was positive, ranging from 370 to 491 copies/ml. Wild type and mutant HBV coexisted in circulation. The mutant virus had mutations in both preS2 and S genes: the preS2 ATG mutated to ATA, and the S gene had a 15-nucleotide repeat insertion in the a determinant. By Blast search in the GenBank, the mutant virus had not been identified before. Nevertheless, the carrier had no signs of liver dysfunction during follow-up period.ConclusionWe identified a novel mutant HBV coexisted with wild type virus in a carrier with negative HBsAg and positive HBeAg and high level of anti-HBs. |
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