Role of αv integrin in osteoprotegerin-induced endothelial cell migration and proliferation |
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| Affiliation: | 1. Department of Disease Control and Molecular Epidemiology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, 1757, Hokkaido 061-0293, Japan;2. Department of Preventive Dentistry, School of Dentistry, Ohu University, Koriyama, Fukushima 963-8611, Japan;1. Departamento de Física, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, IFLP-CONICET, Argentina;2. Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, IFLP-CONICET, Argentina;3. Facultad de Ingeniería, Universidad Nacional de La Plata, IFLP-CONICET, Argentina;1. Departments of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 610041, China;2. Laboratory of Anesthesia & Critical Care Medicine, Translational Neuroscience Center, West China Hospital of Sichuan University, Chengdu, 610041, China;3. The Research Units of West China-Chinese Academy of Medical Sciences (2018RU012), West China Hospital of Sichuan University, Chengdu, 610041, China;4. Research Core Facility, West China Hospital of Sichuan University, Chengdu, 610041, China;1. Department of Dentistry, Far Eastern Memorial Hospital, New Taipei City, Taiwan;2. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan;3. Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan;4. Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan;1. Department of Community Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan;2. Department of Cardiovascular Disease Prevention, Osaka Center for Cancer and Cardiovascular Disease Prevention, Osaka, Japan;3. Department of General Medicine, Nagasaki University Hospital, Nagasaki, Japan;4. Department of Island and Community Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan;5. Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan;1. Department of Surgery, University of Virginia, Charlottesville, Virginia;2. Department of Public Health Sciences, Charlottesville, Virginia |
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| Abstract: | Osteoprotegerin (OPG) is a decoy receptor for the receptor activator of nuclear factor κB ligand (RANKL). However, the role of OPG in the endothelium remains unknown. In this study, we demonstrate that OPG stimulates the proliferation and migration of human microvascular endothelial cells (HMVECs). In addition, we show that treatment with integrin αvβ3 or integrin αvβ5 blocking antibody inhibits endothelial cell migration. In contrast, treatment with anti-αvβ3 antibody or anti-αvβ5 antibody alone did not inhibit OPG-induced proliferation. However, OPG-induced proliferation was inhibited when these antibodies were applied simultaneously. Furthermore, OPG evoked activation of extracellular signal-regulated kinase (ERK) 1/2, which has been linked to integrin αv activity. Taken together, these results suggest that integrins αvβ3 and/or αvβ5 contribute to endothelial cell proliferation and migration induced by OPG. |
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