HPLC-VWD法同时测定丹参及三七混合物中8种有效成分的含量

目的建立HPLC-VWD法同时测定丹参、三七混合物中8种有效成分的含量。方法采用Eclipse XDB-C18(150mm×4.6mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸水梯度洗脱;流速:1.0mL.min-1;柱温:15℃;采用切换波长的方法:在203nm下检测三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1;在286nm下检测丹酚酸B;在270nm下检测隐丹参酮、丹参酮ⅡA、丹参酮Ⅰ。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Re、丹酚酸B、人参皂苷Rb1、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA在相应的浓度范围内与峰面积呈现良好的线性关系,相关系数均大于0.9995;平均回收率97.56%~103.0%,RSD均小于3.0%。结论该方法采用波长切换法同时测定不同吸收波长的化合物,方法简便、重复性好。

Simultaneous quantification of eight compounds in mixture fractions of Salvia miltiorrhiza Bge. and Sanchi by HPLC-VWD

OBJECTIVE A liquid chromatography method with VWD was established to simultaneously quantify eight analytes in mixture fractions of Salvia miltiorrhiza Bge. and Sanchi with different maximum absorption wavelengths. METHODS The chromatographic separation was performed on Eclipse XDB-C18 （150mm× 4.6mm, 5μm） using the gradient elution of aeetonitrile and water containing 0.1 % phosphoric acid at 15℃ . 203,286 and 270nm were switched to determine corresponding analytes. Notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were determined at 203nm. Salvianolie acid B was determined at 286nm. Cryptotanshinone, tanshinone Ⅱ n and tanshinone Ⅰwere determined at 270nm. RESULTS Notoginsenoside R1 ,ginsenoide Rg1 ,ginsenoside Re,salvianolic acid B, ginsenoside Rb1 ,cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ A showed good linearity over the tested ranges （ r ≥0. 9995）. The mean recoveries for eight analytes Were between 97. 56% and 103.0% with RSD below 3.0%. CONCLUSION The method provides a feasible and convenient technique for simultaneous determination of compounds with different maximum UV absorption in one assay by VWD.