Abstract: | Objective:TO discuss how to prepare the liposome of Sculellaria barbata flavones,establish the method of total flavonoids determination,and determate its encapsulation rate.Methods:By using the film dispersion method to prepare the liposome of Sculellaria barbata flavones,and by using the ultracentrifugation to liposome and free drugs, and by using ultraviolet spectrophotometry (UV) to determinate the total content of flavonoids, and determate its encapsulation rate.Results:The Liposome color is dark brown,round or class round,the average particle size was 0.54 microns, uniform distribution,and the average of liposome encapsulation rate is 94.38%, it is in line with the requirements of “Chinese pharmacopoeia”2010 version.Conclusion:The film dispersion method is easy and simple to preparate the Sculellaria barbata flavones Liposome,the operation is easy to grasp,the morphology of liposomes prepared well and high encapsulation rate;The Ultracentrifugation method combined with UV can be accurate, reliable to determinate of the encapsulation rate. |