维生素D受体和雌激素受体基因多态性与慢性牙周炎的相关性

目的 探讨维生素D受体、雌激素受体基因型与慢性牙周炎的相关性,比较宿主携带的各基因型对慢性牙周炎临床指标的影响.方法 将106例中、重度慢性牙周炎患者(牙周炎组)及80名牙周健康者(对照组)作为研究对象,采用Florida探针检测牙周炎患者的牙周探诊深度、临床附着丧失、龈沟出血指数、松动度等临床指标;抽取所有研究对象的前臂静脉血,饱和NaCl法提取DNA后使用聚合酶链反应(PCR)和酶切相结合的方法检测牙周炎组及对照组维生素D受体基因和雌激素受体基因的多态性,Z检验和方差分析进行数据分析.结果 慢性牙周炎患者携带维生素D受体BB基因型的个体百分比为43.4%,健康对照组为30.0%;慢性牙周炎患者携带雌激素受体XX基因型的个体百分比为39.6%,健康对照组为20.0%,差异有统计学意义(P<0.01).同时携带维生素D受体BB基因型、雌激素受体XX基因型的个体牙周状况较携带其他基因型的个体差.结论 维生素D受体等位基因B、雌激素受体等位基因X是中、重度慢性牙周炎的易感等位基因;这两种等位基因的共同携带对慢性牙周炎的病情进展有一定的促进作用.

The relativity between chronic periodontitis and the genetic polymorphisms of vitamin D receptor and estrogen receptor

OBJECTIVE: To investigate the relationship between chronic periodontitis and the genetic polymorphisms of vitamin D receptor gene and estrogen receptor gene. METHODS: Clinical parameters including probing depth, clinical attachment loss, sulcus bleeding index and tooth movement were measured by floride probe. Genomic DNA from peripheral venous blood was extracted with saturant sodium chloride, and PCR-restriction fragment length polymorphism was applied to examine the Apa I, Bsm I, Taq I polymorphisms of the vitamin D receptor genes and the Xba I and Pvu II polymorphisms of the estrogen receptor genes. The results were analyzed by Z-score test and mean square analysis. RESULTS: Forty-three point four percent of chronic periodontitis patients took vitamin D receptor BB genotype, the rate in healthy controls was 30.0%. 39.6% of chronic periodontitis patients took estrogen receptor XX genotype, the rate in healthy controls was 20.0%. The people who took BBXX genotype had the worst periodontal conditions among all chronic periodontitis patients. CONCLUSIONS: Vitamin D receptor allele B and estrogen receptor allele X are susceptible alleles for chronic periodontitis. The synergistic effects of the two receptor susceptible alleles may promote chronic periodontitis.