外源性p27kipl对肺癌细胞系A549的影响

目的：研究外源性p27基因对肺癌细胞系A549的影响。方法，将p27基因重组入腺病毒表达载体pAdCMV中，得到重组腺病毒，感染人肺癌细胞系A549，用激光共聚焦，打点杂交方法检测p27基因在细胞感染前后的表达情况，应用MTT、流式细胞术、细胞软琼脂克隆形成等方法研究p27基因对A549细胞周期，形态、生长等特性的影响。结果：转染p27基因的A549细胞内确有外源性p27基因的表达，p27蛋白表达量明显提高，转染p27基因可使549细胞发生凋亡并发生G1期阻滞。结论：p27基因可诱导肺癌细胞出现凋亡及周期阻滞，为肺癌的基因治疗提供了一个新的思路。

Effect of transfection of p27kipl on lung cancer cells line A549

Objective To observe the suppressive effects of exogenous p27 gene on human lung cancer cell line A549. Methods An adenovirus expression vector (pAd CMV p27) containing 570 bp human full length p27 cDNA was transfected into human lung cancer cell line A549. Expression of exogenous p27 gene was detected by dot blot hybridization and laser co focal system. MTT was adopted to measure the effects of exogenous p27 gene on cell cycle progression and cell features of the infected A549. Results The mRNA and protein expression level of p27 was remarkably increased after transfecting with exogenous p27 gene. The apoptosis of infected A549 occurred and the progression of cell cycle was arrested in G1 phase. Conclusion p27 kipl gene transfer may play a therapeutic role in the treatment of lung cancer. [