| HPLC法测定盐酸苯海拉明乳膏的含量 |
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| 引用本文： | 白林,;范金钊,;蔡乐,;徐风华.HPLC法测定盐酸苯海拉明乳膏的含量[J].中国药物应用与监测,2014(6):348-350. |
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| 作者姓名： | 白林 ;范金钊 ;蔡乐 ;徐风华 |
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| 作者单位： | [1]解放军总医院药品保障中心,北京100853; [2]安徽蚌埠医学院,安徽蚌埠233000 |
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| 基金项目： | 总后制剂标准提高计划重点课题：半固体制剂标准提高系列研究（13ZJZ05） |
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| 摘 要： | 目的：建立测定盐酸苯海拉明乳膏含量的方法。方法：采用HPLC法,色谱柱：Platisil C18柱（250 mm×4.6 mm,5μm）;流动相：乙腈-水-三乙胺（50∶50∶0.5）;检测波长：258 nm;流速：0.8 m L·min^-1;柱温：40℃。结果：盐酸苯海拉明与其它杂质峰分离良好。盐酸苯海拉明在125.0-1 000.0μg·m L^-1范围内与峰面积呈良好的线性关系（r=1.000 0）;盐酸苯海拉明的平均回收率为98.18%,RSD=0.40%（n=9）。结论：本方法简便、准确、重复性好,可用于盐酸苯海拉明乳膏的含量测定。
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| 关 键 词： | 高效液相色谱法 盐酸苯海拉明乳膏 含量测定 |
Content determination of diphenhydramine hydrochloride cream by HPLC |
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| Institution: | BAI Lin, FAN Jin-zhao, CAI Le, XU Feng-hua(1. Department of Pharmaceutical Care, PLA General Hospital, Beijing 100853, China; 2. Bengbu Medical College, Bengbu 233000, China) |
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| Abstract: | Objective： To establish the HPLC method for determining the content of diphenhydramine hydrochloride cream.Methods：HPLC analysis was carried out with Platisil C18column （250 mm×4.6 mm, 5μm）, the mobile phase consisted of acetonitrile-water-triethylamine （50 ： 50 ： 0.5）, the detection wavelength was 258 nm, the flow rate was 0.8 mL·min^-1 and the column temperature was 40℃.Results：The samples were separated well from impurities by this HPLC method. The calibration curve of diphenhydramine hydrochloride was linear in the range of 125.0 – 1 000.0μgmL·min^-1 （r = 1.000 0）. The average recovery and the RSD of diphenhydramine hydrochloride were 98.18%, 0.40% （n = 9）, respectively.Conclusion：This method is accurate and feasible, which can be applied as the quantitative method for the determination of diphenhydramine hydrochloride cream. |
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| Keywords: | HPLC Diphenhydramine hydrochloride cream Content determination |
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