两色金鸡菊醇提物对实验性糖尿病大鼠血糖血脂的影响

目的：考察两色金鸡菊醇提物对实验性糖尿病大鼠血糖血脂的作用。方法：雄性SD大鼠喂养高糖高脂饲料6周后腹腔注射链脲佐菌素（STZ,30 mg·kg^-1）溶液建立实验性糖尿病大鼠模型。将成模大鼠按血糖均衡随机分为模型组、两色金鸡菊醇提物低（0.4 g·kg^-1）、中（0.8 g·kg^-1）、高（1.6 g·kg^-1）剂量组和二甲双胍（0.2 g·kg^-1）组,取同批次健康大鼠作为正常对照组。每天灌胃给药1次。连续给药4周后检测各组大鼠血中糖化血红蛋白（HbAlc）、血清胰岛素（Ins）含量;检测各组大鼠血清中AST、ALT和血脂四项的含量;计算胰岛素抵抗指数（IRI）、胰岛素敏感指数（ISI）和肝脏指数,将空腹血糖（FBG）绘制成曲线,计算AUC值。结果：干预4周后,与模型组相比,两色金鸡菊醇提物中、高剂量组可降低FBG-AUC（P〈0.05）。两色金鸡菊醇提物三个剂量组可明显降低糖尿病大鼠HbAlc、Ins含量（P〈0.01）;显著降低AST、ALT、TC、TG和LDL-C含量（P〈0.01）,同时增加HDL-C水平（P〈0.01）;显著降低IRI和肝脏指数（P〈0.01）,增加ISI（P〈0.05）。结论：两色金鸡菊醇提物具有改善糖尿病大鼠血糖血脂紊乱的作用。

Effect of the alcohol extract from Coreopsis tinctoria Nutt. on blood glucose and lipids in diabetic rat

Objective： To observe the effects of the alcohol extracts from Coreopsis tinctoria Nutt.（ECT） on the blood glucose and lipids in experimental diabetic rats. Methods： Male SD rats were fed with high glucose and high fat diet for 6 weeks, and then streptozotocin（30 mg·kg^-1） was used for inducing diabetic model by peritoneal injection. The diabetic rats were randomly divided into model group, ECT treatment groups（with the doses of 0.4, 0.8 and 1.6 g·kg^-1） and the metformin positive control group（0.2 g·kg^-1）. The normal SD rats from the same batch were used as the normal control group. The rats received daily treatment through intragastric administration for 4 weeks, and then the levels of HbAlc, insulin（Ins）, fasting glucose（FBG）, aspartate transaminase（AST）, alanine aminotransferase（ALT）, triglycerides（TG）, total cholesterol（TC）, high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C） were examined. Insulin sensitivity index（ISI）, liver index and insulin resistance index（IRI） were calculated. The curve was drawn according to the results of FBG, and the area under the cueve（AUC） of FBG was calculated.Results： After 4 weeks, compared with the model group, two ECT treatment groups（with the doses of 0.8 and 1.6 g·kg^-1） reduced the AUC of FBG（P〈0.05）. The three doses of ECT treatment groups significantly decreased the levels of HbAlc, Ins, AST, ALT, TC, TG, LDL-C, IRI and liver index（P〈0.01）, and significantly increased the levels of HDL-C（P〈0.01） and ISI（P〈0.05）. Conclusion： ECT can improve the disturbance of blood lipids, glucose level and insulin resistence in diabetic rats.