旋毛虫病患者血清特异性抗体的检测及其意义

采用ＥＬＩＳＡ方法检测旋毛虫病患血清中各类特异性抗体，结果表明：特异性抗体检出率依次是ＩｇＧ，ＩｇＥ，ＩｇＤ，ＩｇＡ和ＩｇＭ，其中全部病人血清中均有特异性ＩｇＧ，９０．０％的病人检出特异性ＩｇＥ，８６．６７％的病人检出特异性ＩｇＤ，ＩｇＧ是旋毛虫感染免疫中最具保护作用的抗体，检出率高且半衰期长，故首选特异性ＩｇＧ作为免疫诊断的指标；

Determination of the Specific Antibody from Trichinosis Patients and Its Significance

The each class specific antibodies from the trichinosis patients serum was examined by ELISA method. The results showed that the positive rate of specific antibody was IgG, IgE, IgD, IgA, IgM in order. All patients had specific IgG Ab, 90.0% patients had specific IgE, 86.67% patients had specific IgD. Because IgG was the most protective antibody in the infective immunity of T. spiralis and the detection rate of lgG was the highest and the halflife of IgG was long, specific lgG was the first choice as the index of immunodiagnosis. In T. spiralis infection, specific IgE could mediate protective immunity and induce immediate hypersensitivity resulting in immunopathological injury. IgE was a noticeable antibody in T. spiralis infection. The detection rate of specific IgD was high although its immune mechanism was still unclear. This suggested that different class antibodies were determined according to different aim in T. spiralis infection. The examination for each class antibodies may indicate the immune condition of host and the immune effect of vaccine after T.spiralis infection.