铁超载与铁剥夺对Ara-C诱导HL-60细胞凋亡的影响

目的 :探讨铁超载对铁剥夺对Ara C诱导HL 60细胞凋亡的影响。方法 :细胞培养法、台盼蓝活细胞拒染实验、细胞活力测定、光镜形态学观察、DNA琼脂糖电脉及流式细胞仪 (FCM )等方法检测HL 60细胞凋亡。观察不同浓度的三氯化铁 (FeCl3 )、铁剥夺剂 去铁胺 (DFO)等对HL 60细胞的作用 ,选择 10 0 μmol/LFeCl3 和 10 μmol/LDFO与Ara C共同作用于HL 60细胞 ,并以单用Ara C及生理盐水作对照。结果 :①FeCl3 (10 0 μmol/L) +Ara C(10 0 μg/ml) 6h ,12h ,2 4h ,APO %和Sub -G1%低于单用Ara C(10 0 μg/ml)组 ,两组相比有显著差异性 (P <0 0 5 )。②DFO(10 μmol/L) +Ara C(10 0 μg/ml)组APO %、DNA电泳ladder数目 ,FCM检测Sub -G1%均比单用Ara C高 (P <0 0 1)。③等摩尔的DFO与等摩尔FeCl3 共同作用于HL 60细胞 ,结果与生理盐水组相同 ;等摩尔DFO加等摩尔FeCl3 和Ara C(10 0 μg/ml)与单用Ara C(10 0 μg/ml)结果相同。 结论 :铁超载抑制化疗药物Ara C诱导HL 60细胞凋亡作用 ,铁剥夺 (DFO)可促进化疗药物Ara C诱导HL 60细胞凋亡作用。临床上可采用铁剥夺的方法协同治疗白血病 ,白血病患者补铁应慎重

The effects of iron on apoptosis of HL-60 cells induced by Ara-C

Objective: To explore the effects of iron on apoptosis of HL 60 cells induced by Ara C.Methods: HL 60 cells were incubated at 37 ℃ in RPMI 1640 containing 10% heat-inactived fetal bovine serum in an water-saturated 5% CO 2 incubator.apoptosis of HL 60 cells was investigated by cell morphology,flow cytometry assay,DNA gel electrophoresis.Through the experiment,chosing 100 μmol/L FeCl 3?10 μmol/L DFO and Ara C(1 μg/ml?10 μg/ml?100 μg/ml)as the experimental concentration.Results: ①When HL 60 cells cultured with 100 μmol/L FeCl 3+ 100 μg/ml Ara C 6h,12h 24h,APO% were higher than that of Ara C;and sub G1 declined remarkably in comparison with Ara C group ( P <0.01).DNA electrophoresis showed that ladder numbers decreased and the present time of ladder lagged.②Compared 10 μmol/L DFO+ 100 μg/ml Ara C with Ara C,APO% of former was much higher than that of the latter ( P <0.05),Sub G1% of DFO+Ara C group was higher than that of Ara C.③Equimolar concentration of FeCl 3 and DFO+ Ara C,the APO% and Sub G1% was no significant difference with Ara C.Conclusion: Iron overload could inhibited apoptosis of HL 60 cells induced by Ara C,Iron deprivation may promote apoptosis of HL 60 cells induced by Ara C.So in clinical,the iron deprivation could have a place in the treatment of leukemia in conjuncting with other anticancer agents and supplement of iron to leukemic patients shoud be careful.