Foxm1对非小细胞肺癌中 MMP -2、MMP -9和迁移侵袭的影响

目的：研究 Foxm1在非小细胞肺癌中对基质金属蛋白酶2（matrix metalloproteinase -2，MMP -2）、基质金属蛋白酶9（MMP -9）和细胞迁移侵袭能力的影响。方法：Western blot 和 RT - PCR 方法检测 Foxm1表达不同的肺癌细胞中 MMP -2、MMP -9蛋白和 mRNA 的表达水平。采用小干扰 RNA 技术下调 H1299细胞中 Foxm1的表达，Transwell 实验观察 Foxm1对非小细胞肺癌细胞迁移浸润能力的影响。结果：Foxm1高表达的 H1299细胞中，MMP -2、MMP -9在蛋白和 mRNA 的表达水平明显高于 Foxm1低表达的 H1650细胞。而且，Transwell 实验中 H1299细胞和 H1650细胞系穿透到 Transwell 小室下面的细胞数分别为26.8±4.0和7.8±2.0，铺基质胶后穿透到 Transwell 小室下面的细胞数分别为8.7±1.1和2.5±1.4，差异均具有统计学意义。转染特异性 Foxm1 siRNA 后，MMP -2、MMP -9在蛋白和 mRNA 的表达水平随着 Foxm1表达的下降而明显降低。结论：Foxm1可能通过调节 MMP -2、MMP -9的表达，促进非小细胞肺癌细胞的迁移和侵袭。

The effects of Foxm1 on MMP-2, MMP- 9 and metastasis in non - small cell lung cancer

To observe the effects of Foxm1 on MMP - 2,MMP - 9,and the migration and invasion of NSCLC cells. Methods：The expression levels of MMP - 2 and MMP - 9 in two NSCLC cell lines with different Foxm1 expression were detected by Western blot and RT - PCR. We used the small interference RNA technology to knock-down the expression of Foxm1 in H1299 cells. The capabilities of migration and invasion were measured by transwell assay. Results：The expression levels of MMP - 2 and MMP - 9 in H1299 cells were obviously higher than H1650 cells. We selected H1299 and H1650 cells for the following study. At 24 hours after transwell migration and invasion assay,the cell count of each group as H1299 cells and H1650 cells was 26. 8 ± 4. 0 and 7. 8 ± 2. 0,8. 7 ± 1. 1 and 2. 5 ± 1. 4,respectively. Followed by the specific siRNA induced downregulation of Foxm1 in H1299 cells,the ex-pression levels of MMP - 2 and MMP - 9 were decreased obviously. Conclusion：Foxm1 can improve the migration and invasion capabilities of NSCLC cells through the regulation of MMP - 2 and MMP - 9.