Mechanisms of Immunological Response Induced in Cd2f1 Mice by Administration of Semisyngeneic L 1210 Leukemia Cells Treated with Cyclophosphamide |
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Authors: | T Sk rski M Kawalec J Kawiak |
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Institution: |
a Department of Cytophysiology, Medical Centre of Postgraduate Education, Poland |
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Abstract: | CD2F1 mice were immunized against semisyngeneic L 1210 leukemia. Immunization was achieved by four i.p. injections, in weekly intervals, of L 1210 cells treated in vivo twice with 200 mg/kg of cyclophosphamide. The immunized animals survived i.p. challenge with 1000 untreated L 1210 cells that was lethal for nonimmunized mice. The immunity could be abrogated in vivo with anti-mouse thymocyte serum, carrageenan or reserpine, but not by anti-mouse IgG serum, suggesting participation of T lymphocytes and macrophages in the response. Moreover, lymphocytes and macrophages from the peritoneal cavity of immunized mice were cytotoxic in vitro for L 1210 cells. The immunity, at least partially, could be adoptively transferred with peritoneal exudate cells or splenocytes. |
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