Epitope mapping on N-terminal region of taenia solium paramyosin |
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Authors: | Gazarian K G Gazarian T G Solís C F Hernández R Shoemaker C B Laclette J P |
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Institution: | Department of Biotechnology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Aportado Postal 70228, Ciudad Universitaria 04510 D.F., Mexico. karlen@servidor.unam.mx |
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Abstract: | Epitope mapping of the amino-terminal 20aa sequence from Taenia solium paramyosin (TPmy), an immunodominant protein involved in the complex host-parasite relationship in human and porcine cysticercosis is reported. A 12-mer random peptide phage display library was screened with antibodies raised against a synthetic peptide corresponding to the amino-terminal 20aa sequence of TPmy, its highly immunodominant region. In total, 57 clones isolated in two panning conditions were analyzed, of which a single group of 14 sequences found in 25 clones shared a consensus motif showing structural similarity with the antigen Arg10-Thr16 region. |
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