紫花前胡素通过降低OPN表达抑制根尖周炎进程

目的:探讨紫花前胡素对根尖周炎的影响及机制.方法:构建大鼠牙齿根尖周炎用药模型,分为正常对照组、模型观察组和加药处理组,用药处理的大鼠于第1周开始每天腹腔注射紫花前胡素溶液20 mg/kg或等量生理盐水,至第3周末处死.X线及HE染色观察病灶变化;免疫组化检测OPN表达情况.培养并用内毒素及紫花前胡素处理小鼠单核巨噬细...

Decursin Reduces Periapical Inflammation by Inhibiting OPN Expression

Objective:To study the effects and mechanism of decursin in periapical periodontitis.Methods:The rat periapical periodontitis model was established and divided into normal control group,model observation group,and apical periodontitis group.The rats in the apical periodontitis group were equally divided into the decursin group(given decursin,20mg/kg per day)and normal saline group(given the same dose of saline)in the first week,respectively.The rats were sacrificed in the third week.The area of the periapical lesions was analyzed by radiographs and HE staining.The expression of OPN in the alveolar bone was detected using immunohistochemical staining.Mouse mononuclear macrophages RAW264.7 cells were cultured and treated with LPS and decursin.The proliferation and the level of IL-6 were detected by CCK-8 assay and ELISA,respectively.The expression of OPN was examined by western blot.Results:The periapical lesion areas of the control group were remarkably higher than those of the decursin groups.Decursin decreased the protein levels of OPN.The expression of OPN,the proliferation ability,and the level of IL-6 of RAW264.7 cells were increased after treated with LPS.Decursin inhibited these effects.Conclusion:Decursin suppressed the expression of OPN and inhibited the progress of the periapical periodontitis.