骨组织工程3D生物打印的研究进展

近几十年来,骨组织工程在骨缺损修复治疗的应用中获得了飞速的发展,而3D生物打印是近十年来在骨组织工程中一种非常具有前景的技术.传统骨组织工程支架制造方法无法精确控制空间结构,且在支架制作完成后接种细胞也无法控制细胞的均匀分布.尽管3D生物打印作为一类含细胞骨组织工程支架制造技术,以水凝胶类材料作为基础将细胞置入支架中,...

Research progress of 3D bioprinting in bone tissue engineering

In recent decades,bone tissue engineering(BTE)has gained rapid development in the application of bone defect repair,and 3 D bioprinting is a very promising technology of bone tissue engineering in the past ten years.Conventional methods of BTE scaffolds fabrication cannot precisely control the spatial structure.They cannot control the uniform distribution of cells either due to the way seeding cells after scaffolds fabrication are finished.3 D bioprinting(3 DBP),a group of cellular scaffold fabrication technology which places the cells into the scaffolds based on hydrogel-like materials,has solved the problems above.But it still faces many challenges,such as maintaining cell viability after printing,insufficient mechanical strength,osteogenic ability and the most important one—vascularization.In recent years,there have been many studies figuring out these problems through improving bioinks and printing methods.The aim of this review is to introduce the 3 D bioprinting techniques and bioinks used in bone tissue engineering while summarizing some research progress aiming at the current challenges of 3 D BTE scaffolds.We searched CNKI,Wan Fang,Pub Med databases for relevant articles published from 2005 to October 2020.The keywords were"3 D bioprinting;cell-laden 3 D printing;bone tissue engineering;bioink"in Chinese and English,respectively.