DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用

目的 探索二十二碳六烯酸(docosahexaenoic acid,DHA)对人牙龈成纤维细胞(human gingival fibroblasts,HGFs)生物学活性及炎症因子表达的作用.方法 分别采用活死细胞染色法、荧光染色法、流式细胞术观察DHA对细胞活性、细胞形态、细胞周期的影响;DHA预处理HGFs后,利用...

Effects of DHA on biological activity and inflammatory factor expression of human gingival fibroblasts

ObjectiveTo explore the effects of docosahexaenoic acid (DHA) on the biological activity and inflammatory response of human gingival fibroblasts (HGFs).MethodsThe effects of DHA on cell viability, cell morphology and cell cycle were observed by living and dead cell staining, fluorescent staining and flow cytometry, respectively. After pretreated with DHA, HGFs were stimulated with lipopolysaccharide (LPS) from Porphyromonasgingivalis (P. gingivalis) or with heat- inactivated P. gingivalis. Then the gene and protein expression of interleukin-6 (IL-6), IL-8 and IL-1β was detected by quantitative PCR and ELISA, respectively. The effect of DHA on heme oxygenase-1 (HO-1) gene and protein expression was also observed by quantitative PCR and western blot, respectively. Results200 μmol/L DHA reduced the cell activity of HGFs, but 100 μmol/L DHA had no effect on cell viability and morphology, and had no significant effect on cell cycle (P>0.05); DHA inhibited the expression of IL-6, IL-8, IL-1β mRNA and IL-6, IL-8 protein induced by P. gingivalis LPS or heat- inactivated P. gingivalis in a concentration-dependent manner (P<0.05); DHA significantly induced HO-1 mRNA and protein expression (P<0.05).ConclusionDHA significantly inhibited the inflammatory response of HGFs without affecting the biological activity. The anti-inflammatory effect may be related to the induction of HO-1.