| 鸟苷酸交换因子Trio通过调控神经嵴细胞迁徙影响颅颌面发育 |
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| 引用本文： | 孟丽,郭舒瑜,刘浩洁,袁礼婵,顾嘉雯,马俊青. 鸟苷酸交换因子Trio通过调控神经嵴细胞迁徙影响颅颌面发育[J]. 口腔生物医学, 2021, 12(1): 17-20. DOI: 10.3969/j.issn.1674-8603.2021.01.004 |
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| 作者姓名： | 孟丽 郭舒瑜 刘浩洁 袁礼婵 顾嘉雯 马俊青 |
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| 作者单位： | 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029;南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029;南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029;南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029;南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029;南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院正畸科,江苏 南京210029 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目;国家自然科学基金 |
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| 摘 要： | 目的:研究鸟苷酸交换因子Trio在胚胎发育过程中对神经嵴细胞的影响.方法:将trio反义寡核苷酸通过显微注射入斑马鱼胚胎内,抑制其trio功能;在斑马鱼胚胎发育96 h时使用体式显微镜观察神经嵴细胞衍生物下颌骨的发育情况,120 h时通过阿尔辛蓝染色观察颅颌面部软骨的发育状况.利用转基因斑马鱼(sox10:EGFP)特...
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| 关 键 词： | Trio 神经嵴细胞 迁徙 颅颌面部发育 斑马鱼 |
Guanine nucleotide exchange factor Trio effects craniofacial development by regulating neural crest cells migration |
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| MENG Li,GUO Shuyu,LIU Haojie,YUAN Lichan,GU Jiawen,MA Junqing. Guanine nucleotide exchange factor Trio effects craniofacial development by regulating neural crest cells migration[J]. Oral Biomedicine, 2021, 12(1): 17-20. DOI: 10.3969/j.issn.1674-8603.2021.01.004 |
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| Authors: | MENG Li GUO Shuyu LIU Haojie YUAN Lichan GU Jiawen MA Junqing |
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| Affiliation: | (Jiangsu Key Laboratory of Oral Diseases,Department of Orthodontics,Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China) |
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| Abstract: | Objective: To investigate the effect of Rho GEF Trio on neural crest cells during embryonic development. Methods:Trio function was inhibited by microinjection of morpholino(MO)into zebrafish embryos,the development of mandibular and neural crest cells derivative was observed at 96 h through stereo microscope,and the development of craniomaxillofacial cartilage was observed at 120 h by Alcian blue staining. The effect of trio on the neural crest cells migration path of zebrafish was observed by using the neural crest cell specific labeling technique of transgenic zebrafish(sox10: EGFP)by means of Timelapse. Trio knockdown neural crest cells were cultured in vitro to observe the effect of Trio on the migration of neural crest cells through measuring the wound area(%)after migration in wound healing assay. Results: When trio function was inhibited,zebrafish mandibular development was insufficient,the Meckel’s cartilage became retrusive,and the distance of neural crest cells migration decreased. In vitro,the wound area after neural crest cell migration increased in the Trio knockdown group. Conclusions: Trio may promote craniofacial development of neural crest origin by regulating the neural crest cells migration. |
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| Keywords: | Trio neural crest cells migration craniofacial development zebrafish |
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