精氨酸联合生物活性玻璃对乳牙釉质龋再矿化的研究

目的 研究精氨酸联合生物活性玻璃对乳牙釉质早期龋的再矿化性能。方法 将乳牙人工龋样本随机分为5组：①6%生物活性玻璃组 ②6%生物活性玻璃+1%精氨酸组 ③6%生物活性玻璃+2%精氨酸组 ④6%生物活性玻璃+4%精氨酸组 ⑤去离子水组，并进行14天的pH循环。显微硬度仪检测釉质表面硬度，电镜观察釉质的表面形貌，能谱分析仪分析表面的钙磷含量及钙磷比。结果 各组表面硬度恢复率（%）分别为(54.49±2.44)、（65.95±4.11）、（67.00±2.03）、（63.73±1.64）、（8.46±2.63）；各组均形成新的钙化层，添加2%及4%精氨酸组的釉质表面最为平坦；精氨酸协同组钙磷含量及钙磷比均显著高于生物玻璃组，其中2%及4%精氨酸组钙磷比与正常釉质相似。结论 精氨酸与生物活性玻璃对乳牙早期龋的再矿化有协同作用，其中添加2%精氨酸组综合能力最佳。

Effects of arginine combined with 45S5 bioglass on the remineralization of early carious lesions in deciduous teeth:An in vitro study

Objective To evaluate the effect of arginine(Arg) combined with Bioglass(BAG) on the remineralization of early caries lesions in deciduous enamel . Methods Caries-like enamel lesions of were assigned to 5 experimental groups : (1) 6%BAG, (2) 6%BAG+1%Arg, (3) 6%BAG+2%Arg, (4) 6%BAG+4%Arg, (5) deionized water and transported to a 14 day's pH-cycline. Vickers hardness test evaluated the mechanical properties through enamel microhardness recovery ratio (% REMH). Surface morphologies along with Ca (Wt%)、P (Wt%),、and Ca/P atomic ratio were observed using scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. Results % REMH processed by each group were (54.49±2.44), (65.95±4.11), (67.00±2.03), (63.73±1.64b), (8.46±2.63c); a newly formed layer was found in all groups, among which the 2% and 4% arginine combined groups showed the flattest; the Ca(wt%)、P (wt%) and Ca/P ratio were significantly higher in combination groups than bioglass group, and 2% and 4% arginine combination groups showed the similar Ca/P to the normal enamel. Conclusion Arginine and bioactive glass have a synergistic effect on the remineralization of deciduous teeth, and the 2% arginine combined with 6% bioglass showed the best comprehensive ability.