SGK1在鼻咽癌中的表达及其对鼻咽癌细胞生物学特性的影响

DOI:10.11798/j.issn.·鼻咽癌专栏·基金项目：第目的探讨SGK1在鼻咽癌（NPC）中的表达及其对肿瘤细胞存活、增殖和迁移的影响。方法采用免疫组织检测NPC组织和慢性鼻咽炎组织中SGK1的表达。使用lipofectamine 2000瞬时转染NPC细胞系HNE 1，Western blot检测siRNA干扰组、阴性对照组及未转染组细胞中的SGK1表达，筛选SGK1沉默效果最佳的NPC细胞。CCK8法检测转染细胞的增殖能力，Transwell细胞迁移实验检测SGK1沉默对细胞迁移能力的影响，流式细胞术检测其凋亡水平。结果NPC组织中SGK1的表达明显高于慢性鼻咽炎组织。Western blot结果显示转染SGK1 siRNA后HNE 1细胞中SGK1蛋白水平明显下降。SGK1基因沉默后，HNE 1细胞表现出较强的细胞增殖和迁移抑制作用。此外，沉默SGK1后，HNE 1细胞的凋亡率增加。结论SGK1在NPC组织中呈高表达。沉默SGK1基因可抑制NPC细胞系HNE 1的增殖、迁移和存活能力。

The expression of SGK1 and its effect on biological characteristics of nasopharyngeal carcinoma cell

ObjectiveTo explore the expression of serum and glucocorticoid inducible kinase 1 (SGK1) in nasopharyngeal carcinoma (NPC) and its impacts on tumor cell survival, proliferation and migration.MethodsImmunohistochemistry was performed to detect the expression of SGK1 protein in NPC tissue and chronic nasopharyngitis tissue. After transfected using lipofectamine 2000, HNE 1 cells with the best silence effect were selected by detecting the expression of SGK1 in cells of SGK1 siRNA induced group, negative control group and non transfection group using western blotting. CCK 8 assay, transwell assay, and flow cytometry were performed to study the effects of SGK1 on the proliferation, migration, and apoptosis of HNE 1 cells.ResultsThe expression of the SGK1 in NPC was increased compared to the chronic nasopharyngitis tissue. The protein level of SGK1 got evidently decreased after transfection of SGK1 siRNA. After gene silencing of SGK1, HNE 1cells showed strong inhibition of cell proliferation and migration. In addition, the apoptosis rate increased in HNE 1 cells after SGK1 knockdown.ConclusionsSGK1 is highly expressed in NPC tissue. Silencing SGK1 suppresses the survival, proliferation and migration of NPC cells.