二陈汤及桃红四物汤对非酒精性脂肪肝CYP2E1活性影响的实验研究

目的:观察二陈汤及桃红四物汤对大鼠非酒精性脂肪肝模型肝细胞色素酶CYP2E1活性的影响,探讨二陈汤及桃红四物汤在非酒精性脂肪肝治疗中的作用。方法:制备非酒精性脂肪肝大鼠动物模型,用二陈汤及桃红四物汤分别治疗。实验结束后,检测肝细胞微粒体蛋白含量以及CYP2E1活性。结果:脂肪肝大鼠存在明显的肝细胞微粒体蛋白降低以及CYP2E1活性增强。与模型组相比,二陈汤对脂肪肝大鼠肝细胞微粒体蛋白具有明显升高作用(P<0.05);而对CYP2E1活性则具有明显降低作用(P<0.01)。桃红四物汤也有一定作用趋势,但无统计学意义。结论:二陈汤不仅能够改善高脂血症状态,也能降低CYP2E1活性,防治因此而导致的过氧化损伤过程,从多方面治疗非酒精性脂肪肝。

Experimental study on effect of Erchen decoction and Taohongsiwu decoction on CYP2E1 activities in non-alcoholic fatty liver rats

Objective: To observe the therapeutic effects of Erchen decoction, Taohongsiwu decoction on CYP2E1 activity and microsome protein content in non-alcoholic fatty liver rats.Methods: Experimental animal model of non-alcoholic fatty liver rats were established , then treated with Erchen decoction, TaohongSiwu decoction.Microsome protein content and cyp2e1 activity are tested after treatment.Results: Microsome protein content of non-alcoholic fatty liver rats reduced and cyp2e1 activity increased. Compared with model group, in Erchen decoction group, the microsome protein content in hepatocyte increased significantly (P<0.01), and the cyp2e1 activity decreased obviously (P<0.01). cyp2e1 activity in Taohongsiwu group decreased also. Conclusion: Erchen decoction can not only improve hyperlipidemia, but also can reduce cyp2e1 activity, prevent and therapy oxidative injury. Its therapeutic effects on NAFLD are rich.