黄芪注射液和有氧运动对一次力竭运动大鼠外周血T淋巴细胞亚群的影响

目的:探讨黄芪注射液和有氧运动对一次力竭运动大鼠外周血T淋巴细胞亚群的影响,为改善力竭运动引起的免疫功能低下寻找有效的干预措施。方法:将50只大鼠随机均分为5组:对照组、一次性力竭运动组(取材前做一次力竭运动)、有氧运动+一次性力竭运动组(有氧运动4周后做一次力竭运动)、服药+一次性力竭运动组(灌服黄芪注射液4周后做一次力竭运动)、服药+有氧运动+一次性力竭运动组(灌服黄芪注射液并做有氧运动4周后做一次力竭运动),每组10只。经过4周的实验后,记录力竭运动时间,用流式细胞术测大鼠外周血CD4+和CD8+百分率,并测定实验前后大鼠体重。结果:一次力竭后大鼠CD4+T百分率和CD4+/CD8+比例显著降低(P0.01),CD8+T百分率显著升高(P0.01),黄芪注射液和有氧运动均预防T淋巴细胞亚群的这种变化;有氧+力竭组、服药+力竭组和服药+有氧+力竭组大鼠的力竭时间较一次力竭组大鼠长(P0.01),并且服药+有氧+力竭组大鼠的力竭时间较有氧+力竭组、服药+力竭组大鼠长(P0.01)。结论:黄芪注射液和有氧运动均可提高大鼠运动能力,可预防力竭运动大鼠免疫功能降低,两者配合在提高运动能力方面具有协同作用。

Effect of Radix Astragali Injection and Aerobic Exercise on Quantities of Peripheral T Lymphocyte Subsets in Rats Performing once Exhausting Exercise

Objective: In order to search potent intervening means for low-level exercise-associated cellular immune capacities, effect of Radix Astragali injection and Aerobic exercise on quantities of peripheral T lymphocyte subsets in rats was observed. Method: 50 Wister Rats were randomLy divided into 5 group: quiet control group(n=10), once exhausting exercise group(once exhausting exercise before drawning materials)(n=10), Aerobic exercise and once exhausting exercise group(once exhausting exercise after aerobic exercise for four weeks)(n=10), Radix Astragali injection and once exhausting exercise group(once exhausting exercise after oraling Radix Astragali injection for four weeks)(n=10), Radix Astragali injection and Aerobic exercise and once exhausting exercise group(once exhausting exercise after oraling Radix Astragali injection and for aerobic exercise four weeks)(n=10). After 4 weeks test, time of exhaustive exercise were record,percentage of peripheral T Lymphocyte subsets in circulating blood were tested by FCM,and body weight were measured before and after experiment. Result: Afer exhausting exercise, percentage of CD4⁺ and the ratio of CD4⁺ to CD8⁺ in Lymphocyte subsets,as well as were lower(P<0.01),percentage of CD8⁺ as well as were higher than that in normal control group(P<0.01).The change was prevented by Radix Astragali injection and aerobic exercise; the exhausting exercise time of aerobic exercise and once exhausting exercise group,astragali injection and once exhausting exercise group,Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than once exhausting exercise group(P<0.01),and the exhausting exercise time of Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than aerobic exercise and once exhausting exercise group,Astragali injection and once exhausting exercise group(P<0.01). Conclusion: Radix Astragali injection and aerobic exercise may improve exercise capacity and preven immunosuppression in rats after exhaustive exercise in rats, enhance effect after combinating the two means.