| 曲古抑菌素A对人胃癌SGC-7901细胞凋亡及其RASSF1A基因表达的影响 |
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| 引用本文： | 廖爱军,苏琦,童汪霞,成龙,肖伟升,陈娇艳.曲古抑菌素A对人胃癌SGC-7901细胞凋亡及其RASSF1A基因表达的影响[J].肿瘤防治研究,2010,37(3):281-283. |
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| 作者姓名： | 廖爱军 苏琦 童汪霞 成龙 肖伟升 陈娇艳 |
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| 作者单位： | 1.421001 湖南衡阳,南华大学附一医院消化内科;2.南华大学肿瘤研究所 |
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| 基金项目： | 湖南省科研计划重大专项基金，湖南省卫生厅科学研究项目 |
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| 摘 要： | 目的探讨组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)对人胃癌SGC-7901细胞凋亡及其RASSF1A基因表达调控的影响。方法用0.1、0.3、1.0μmol/L TSA分别处理人胃癌SGC-7901细胞，流式细胞仪检测细胞凋亡率，RT-PCR、Western blot分别检测RASSF1A mRNA和蛋白表达水平。结果流式细胞仪分析表明，不同浓度TSA可诱导SGC-7901细胞凋亡，并呈浓度及时间依赖性（P<0.05）。TSA干预前SGC-7901细胞无RASSF1A mRNA及蛋白表达，而不同浓度TSA处理SGC-7901细胞后，RASSF1A mRNA及蛋白表达分别上调，呈浓度及时间依赖性(P<0.05)。结论TSA诱导SGC-7901细胞凋亡可能与RASSF1A基因异常乙酰化状态恢复导致基因重新表达有关。
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| 关 键 词： | TSA 胃癌 凋亡 RASSF1A基因 组蛋白乙酰化 |
| 收稿时间： | 2008-10-18 |
| 修稿时间： | 2009-5-5 |
Effects of TSA on Apoptosis and Expression of RASSF1A Gene in Human Gastric Cancer SGC-7901 Cells |
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| LIAO Ai-jun,SU Qi,TONG Wang-xia,CHENG Long,XIAO Wei-sheng,CHEN Jiao-yan.Effects of TSA on Apoptosis and Expression of RASSF1A Gene in Human Gastric Cancer SGC-7901 Cells[J].Cancer Research on Prevention and Treatment,2010,37(3):281-283. |
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| Authors: | LIAO Ai-jun SU Qi TONG Wang-xia CHENG Long XIAO Wei-sheng CHEN Jiao-yan |
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| Institution: | 1.Department of Gastroenterology,The First Affiliated Hospital,University of South China,Hengyang 421001,China；2.Cancer Research Institute,University of South China |
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| Abstract: | Objective To investigate the effects of the histone deacetylation inhibitor,trichostatin A (TSA) on the expression of RASSF1A gene in human gastric cancer cell line.Methods Gastric cancer SGC-7901 cells were treated with different concentration of TSA.The apoptosis of the cells were detected by flow cytometry.RT-PCR and Western blot were applied to detect the RASSF1A mRNA and protein expression levels,respectively.Results Flow cytometry analysis showed that the apoptosis rate in SGC-7901 cells went up signi... |
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| Keywords: | TSA TSA Gastric cancer Apoptosis RASSF1A gene Historic acetylation |
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