猪松质骨支架兔体内移植后周围组织Th1和Th2细胞因子的变化

目的观察猪松质骨支架植入后周围组织Th1和Th2细胞因子的变化,为进一步改进材料和临床应用提供实验依据。方法采用物理化学方法对猪松质骨进行处理得到猪松质骨支架,将松质骨支架植入兔背部近头侧皮下,分别于移植后1、2、4、8、12周5个时间点作支架周围组织的组织学检查（HE）及支架周围组织内Th1和Th2细胞因子mRNA检测（RT-PCR）。结果移植术后1周,植入物周围组织光学显微镜下可见大量炎性细胞浸润,以淋巴细胞和单核细胞为主,2周以后炎性细胞浸润逐渐减轻,4周时未见明显炎性细胞浸润,术后12周,组织内炎性细胞罕见。RT-PCR检测Th1细胞因子IL-2、IFN-γ mRNA1周时就有较高水平表达,2-8周逐渐下降,12周时降至低水平;Th2细胞因子IL-4、IL-10 mRNA1周时低水平表达,2周、4周时表达水平表达逐渐上升,8-12周时维持较高水平表达。结论猪松质骨支架异种松质骨支架材料无明显的免疫原性,植入后表现为一过性或暂时性的轻度炎性细胞反应。

Change of the expression level of Th1 and Th2 cytokines around the xenogeneic cancellous bone scaffold after transplation: an experiment in rabbits

OBJECTIVE: To observe the dynamic immunity of the porcine cancellous bone scaffold(PCBS) after transplantation and provide experimental evidences for amending and clinical use. METHODS: The PCBS was gained through physical and chemical disposals to porcine cancellous bone and was implanted subcutaneously in rabbit. The histological investigation and the expression of Th1 (such as IL-2, IFN-gamma) and Th2 (such as IL-4, IL-10) mRNA were measured by HE, RT-PCR respectively in 1,2,4,8,12 week postoperation. RESULTS: HE revealed: Inflammation cells (lymphocytes and monocytes) were shown abundantly in the surrounding tissue in 1 week and were decreased gradually since 2 week. At 12 week, there were few inflammation cells in the surrounding tissue. RT-PCR revealed: The mRNA expression level of Th1 cytokines such as IL-2, IFN-gamma was high in 1 week and began to fall since 2 week. At 12 week, the mRNA expression level of Th1 cytokines was low; The mRNA expression level of Th2 cytokines such as IL-4, IL-10 was low in 1 week, rase gradually since 2 week and reached tiptop in 8 week. At 12 week, the mRNA of Th2 cytokines maintained high level expression. CONCLUSIONS: The porcine cancellous bone scaffold excitate immunoreaction was transitory inflammation after implantaion.