树突状细胞浸润对原发性肝癌生物学行为和预后的影响

目的:研究原发性肝癌组织中树突状细胞(DC)浸润与肝癌临床病理特征、生物学行为、预后之间的关系,明确DC浸润对肝癌发生发展及预后的影响。方法:用免疫组化(即用型两步法SuperveisionTM)法检测60例原发性肝癌术后组织标本中DC标志蛋白S-100和增殖细胞核抗原(PCNA)的表达;以原位末端标记技术(TUNEL)检测肝癌细胞凋亡比例。结果:高分化肝癌组织中DC浸润数量高于低分化肝癌(P<0.05);在无门静脉侵犯或淋巴结转移的肝癌组织中DC浸润数量显著高于有门静脉侵犯或淋巴结转移的肝癌组织(P<0.05);DC高浸润组的晚期肝癌比例低于DC低浸润组(P<0.05);DC高浸润组预后好于DC低浸润组(P<0.05)。DC浸润与增殖细胞核抗原标记指数(PCNA-LI)呈负线性相关(r=-0.337,P<0.01),与凋亡细胞指数(AI)呈正线性相关(r=0.435,P<0.01)。结论:DC浸润与肝癌的生物学行为密切相关,DC在肝癌的发生发展和预后中有重要作用,抑制肝癌细胞增殖、促进肝癌细胞凋亡是其可能的作用机制之一。

Influence of Dendritic Cell Infiltration on Prognosos and Biologic Characteristics of Primary Liver Cancer

Objective To study the relationship between dendritic cells(DC) infiltration and clinicopathologic parameters,biologic characteristics and prognosis of primary liver cancer(PLC).Methods The PCNA labeling index(PCNA-LI) and density of tumor infiltrating DC in PLC tissues were detected by immunohistochemical stain(using antibody against PC-10 protein and S-100).The rate of apoptotic cell(apoptotic index,AI) in 60 PLC tissues was analyzed by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling(TUNEL) method.Results The number of DC infiltration in the groups with well differentiation and without portal vein invasion or metastasis were significantly higher than those in the groups with poor differentiation and with portal vein invasion or metastasis.The prognosis of patients with high DC infiltration was significantly better than those with low ones.A positive correlation was observed between DC infiltration and AI(r=0.435,P<0.01) whereas a negative correlation between DC infiltration and PCNA-LI(r=-0.337,P<0.01).Conclusion The infiltrating DC in and around tumor,representing the local immune status of the host,may play an important role in immunological defense mechanism of host versus tumor.DC may inhibit the proliferation and induce the apotosis of the tumor cells,thus affecting the clinical features and improve the prognosis of PLC.