高效液相色谱法测定妇月康胶囊中阿魏酸含量 |
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引用本文: | 兰作平,覃树勇,吴朝荔,韩永会,曾正渝. 高效液相色谱法测定妇月康胶囊中阿魏酸含量[J]. 中国药业, 2008, 17(15): 40-41 |
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作者姓名: | 兰作平 覃树勇 吴朝荔 韩永会 曾正渝 |
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作者单位: | [1]重庆医药高等专科学校,重庆400030; [2]贵州神奇制药有限公司技术部,贵州贵阳550004 |
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摘 要: | 目的用高效液相色谱(HPLC)法测定妇月康胶囊中阿魏酸含量。方法采用Kromasil C18(250mm×4.6mm,5μm),流动相为乙腈-甲醇-0.1%磷酸(8:17:75),检测波长为320nm。结果阿魏酸质量浓度在4—32μg/mL范围内与峰面积线性关系良好,平均回收率为99.80%,RSD=1.26%(n=6)。结论HPLC法简便、快速、准确,可用于妇月康胶囊的含量测定。
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关 键 词: | 高效液相色谱法 含量测定 阿魏酸 妇月康胶囊 |
Determination of Ferulic Acid in Fuyuekang Capsules by HPLC |
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Affiliation: | Lan Zuoping, Qin Shuyong, Wu Chaoli, Hart Yonghui, Zeng Zhengyu (1. Chongqing Pharmaceutical College, Chongqing, China 400030; 2. Department of Technique, Guizhou Shenqi Pharmaceutical Co., Ltd., Guiyang, Guizhou, China 550004) |
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Abstract: | Objective To establish a HPLC method for the determination of ferulic acid in Fuyuekang Capsules. Methods The HPLC analysis was carried out with Kromasil C18 column and acetonltrile-methanol-0. 1% phosphoric acid (8:17:75) as the mobile phase. The wavelength was 320 nm. Results There was a good linear relationship between the absorption area value and the concentration in the range of 4 - 32 μg/mL. Conclusion The method is simple,accurate and reproducible. It can be used in the quality control of Fuyuekang Capsules. |
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Keywords: | HPLC: content determination ferulic acid Fuyuekang Capsules |
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