Raf激酶抑制蛋白在宫颈癌中的表达及其临床意义

目的 探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)在宫颈癌中的表达及其临床意义.方法 应用免疫组织化学方法检测RKIP在正常官颈组织、官颈上皮内瘤变组织、官颈癌及其转移淋巴结组织的表达,并在体外应用RT- PCR及Western印迹方法检测RKIP mRNA及蛋白在4种不同来源的宫颈癌细胞系中的表达情况,探讨其与官颈癌临床病理学特征的关系.结果 RKIP在宫颈癌转移淋巴结中的阳性表达率低于宫颈癌组织；RKIP在宫颈癌组织的表达低于正常宫颈组织及宫颈上皮内瘤变组织,差异有统计学意义(x2 =9.211,P＜0.05)；而RKIP在正常官颈组织及官颈上皮内瘤变组织的表达差异无统计学意义(x2=0.805,P＞ 0.05)；RKIP在宫颈癌组织中的表达与淋巴结转移有关(x2=10.341,P＜0.05),与肿瘤的病理类型、分化程度及临床分期无关(x2分别为0.190、4.101、1.402,P＞0.05).RT- PCR及Western印迹结果显示RKIP在高侵袭性宫颈癌细胞系Caski中的表达低于其他各组(x2=4.08,P＜0.05).结论 RKIP基因在宫颈上皮内瘤变向官颈癌转变中可能起重要作用,是官颈癌发生淋巴结转移的重要标记.

Purpose to study the expression of RKIP in cervical cancer and its clinical implication

Objective To investigate the expression of the RKIP protein in the cervical tisssue and the relationship of the RKIP with the clinical and pathological of the cervical cancer.Methods Immunohistochemical study was used to study the expression of the RKIP protein in the normal cervical tissue,cervical intraepithelial neoplasia,cervical cancer and its metastatic lymph nodes ; RT-PCR and Western Blot methods were also used to study the expression of the RKIP in the four kinds of the cervical cancer cells,we analysed the the relationship of the RKIP with the clinical and pathological of the cervical cancer.Results RKIP expression level was lower in cervical cancer than in norma cervical tissue and cervical intraepithelial tissue (x2 =9.211,P ＜ 0.05 ).There was no difference in the expression of the RKIP between the normal cervical tissue and cervical intraepithelial tissue (x2 =0.805,P ＞ 0.05 ).RKIP expression had a correlation with lymph node metastasis (x2 =10.341,P ＜ 0.05 ).No significant correlation were observed between RKIP expression and clinical stage,microscopic subtype or pathology differentiation grade,(x2 =0.190、4.101、1.402,P ＞0.05).The result of the RT-PCR and western blot showed the RKIP expression level was lowest in high investive Caski cervical cancer cells (x2 =4.08,P ＜ 0.05 ).Conclusion RKIP may play a role in the genesis and metastasis of cervical cancer.Reduction of RKIP expression is a hallmark of metastatic disease in human cervical cancer.