八聚体转录因子4蛋白表达与非肌层浸润性膀胱癌的关系

目的 探讨八聚体转录因子4(Oct4)蛋白在非肌层浸润性膀胱癌中的表达情况,并分析其表达与患者临床病理特征及预后的关系.方法 采用免疫组化生物素酶标法检测87例非肌层浸润性膀胱移行细胞癌和15例癌旁正常组织中Oct4蛋白的表达情况,并结合临床资料分析Oct4蛋白的表达在膀胱癌患者中的意义.结果 正常组织中Oct4蛋白的表达水平明显低于肿瘤组织(P＜0.01).G1级、G2级、G3级膀胱癌的Oct4蛋白阳性表达率分别为40.7％、69.4％、91.7％,差异有统计学意义(P＜0.01).所有患者随访3～78个月,其中63例出现复发,复发组与未复发组中Oct4蛋白的阳性表达率分别为77.8％和37.5％,差异有统计学意义(P＜0.0l)；21例复发患者出现进展,进展组与未进展组中Oct4蛋白的阳性表达率分别为71.4％和65.2％,差异无统计学意义(P＞0.05).Oct4蛋白的表达与患者的年龄、性别、肿瘤的数目及大小无明显相关性(P＞0.05).结论 Oct4蛋白检测有助于早期发现膀胱肿瘤,并可用来判断肿瘤的恶性分化程度及检测患者术后的复发情况.

Expression of Oct4 protein in non-muscle-invasive bladder cancer

Objective To investigate the expression of Oct4 protein and analyze its correlation with the clinic pathological features and prognosis of non-muscle-invasive bladder cancer.Methods The oct4 protein expression was assessed by immunohistochemical analysis in 87 specimens of bladder transitional cell carcinoma and 15 specimens of adjacent normal tissues.A correlation between Oct4 and clinic pathological features was analyzed.Results The positive rate of Oct4 protein was significantly higher in bladder cancer than that in normal bladder tissue (P＜0.01).The positive rate of Oct4 protein was 40.7％ in G1 bladder cancer,69.4％ in G2 bladder cancer and 91.7％ in G3 bladder cancer,and the differences was significant (P＜0.01).All patients were followed up for 3-78 months,and 63 of them relapsed.The expression of Oct4 protein was significantly higher in patients of recurrence than in non-recurrence (77.8％ ∶ 37.5％,P ＜ 0.01 ).21 patients of recurrence were in progression,and the expression of Oct4 protein had no significant differences between patients of progression and non-progression (71.4％ ∶65.2％,P ＞0.05).The positive rate of Oct4 protein was not related with gender,age,tumor number and size (P ＞0.05).Conclusion The detection of Oct4 protein is in favor of early detection of bladder tunor,estimation the degree of differentiation and surveillance for recurrence of superficial bladder cancer.