己酮可可碱对创伤失血性休克大鼠器官血流量及存活率的影响

目的探讨己酮可可碱(PTX)对创伤失血性休克大鼠肝、脾、肾血流量和存活率的影响及其机制。方法选择健康雄性Wistar大鼠30只,随机分为正常组、对照组和治疗组各10只。对照组和治疗组大鼠经右股动脉10min内放血至平均动脉压(MAP)为(40±2.0)mm Hg,此后间断放血或回输血以维持此血压水平1.5h,以4倍失血量的乳酸复方乳酸钠(LR)复苏休克大鼠,复苏量于1h内经右颈外静脉由微量泵均匀泵入;治疗组大鼠于复苏45min后经右颈外静脉插管按30mg/kg加用PTX治疗,并以此时作为时相起点,治疗剂量PTX由生理盐水配成4mL,其半量于5min内均匀泵入,余量即2mL于90min由微量泵均匀泵入;对照组相应时间给予等量生理盐水。比较3组大鼠4.5、6.5h肝、脾及肾脏器官的血流量及8、16h的成活数。结果治疗组大鼠肝、脾及肾脏器官的血流量明显高于对照组(P<0.05)。结论 PTX显著提高创伤失血性休克大鼠LR复苏后肝、脾、肾脏器官的血流量,增加实验动物的存活率。

Effects of pentoxifylline on organ blood flow and survival rate in rat traumatic-hemorrhagic shock

Objective To evaluate the effects of pentoxifylline(PTX) on the organ blood flow and the survival rate in rat traumatic-hemorrhagic shock.Methods 30 male Wistar rats were randomly divided into 3 groups:normal group(SS),control group(LR) and therapy group(PTX),10 cases in each group.All rats in the three groups were wounded by laparotomy and fracture of left femur.The rats in control group and therapy group were bled from the right femoral artery to reduce the mean arterial pressure(MAP) to(40±2.0)mm Hg with in 10 min after trauma.MAP was kept at this level for 1.5 h and then the rats were resuscitated with lactated Ringer′ s solution(LR),four times of shed blood volume,through the right external jugular vein within 1 h.At 15 min before the end of resuscitation,the rats in therapy group were infused PTX(30 mg/kg body weight) through the vein within 95 min during and after resuscitation,simultaneously,the rats in control group were given the same volume of normal saline solution during the same period.The organ blood flow of liver,kidney and spleen were determined respectively at 4.5,6.5 h after PTX treatment.The rat survival rates at 8,16 h were calculated.Results The experiment results indicated that the organ blood flow(liver,spleen and kidney) was significantly higher in PTX group than in LR group(P<0.05).Conclusion PTX restores the blood flow of liver,kidney and spleen and increases the survival rate of animals after PTX administration.