系统性硬皮病患者循环内皮祖细胞数量变化的初步研究

目的分析系统性硬皮病患者外周血内皮祖细胞数量的变化。方法选择2010年11月至2011年6月该院风湿免疫科就诊的女性系统性硬皮病(SSc)患者20例作为实验组,同期健康体检者20例为对照组,取外周血20mL,密度梯度离心法分离单个核细胞,以CD133/CD34、CD133/血管内皮细胞生长因子受体2(VEGFR2)双荧光标记鉴定细胞,CD34/CD133/VEGFR2三荧光标记流式检测内皮祖细胞数量。结果实验组患者外周血内皮祖细胞表达阳性率(3.18±1.97)%较对照组(20.56±4.37)%低(P<0.05)。结论系统性硬皮病患者外周血内皮祖细胞数量减少。

Preliminary study on change in number of endothelial progenitor cells in peripheral blood of systemic scleroderma patients

Objective To analyze the change of number of the endothelial progenitor cells(EPCs) from peripheral blood(PB) in systemic scleroderma(SSc) patients.Methods 20 female patients with SSc in the rheumatism and immunology department of our hospital from November 2010 to June 2011 were selected as the experimental group and 20 healthy females with physical examination were as the control.20 mL PB was collected for isolating mononuclear cells(MNCs) by the density gradient centrifugation method,and EPCs were identified by analysis of both the fluorescence expression of CD34 and CD133,or both CD133 and VEGFR2.The number of EPCs was flow-cytometrically determined.Results Compared with the control group,the positive expression rate of EPCs was significantly reduced in the patients of the experimental group(3.18±1.97)% vs.(20.56±4.37)%,P<0.05.Conclusion The number of EPCs is significantly reduced in SSc patients.