超声波治疗兔膝骨关节炎对MMP-1和MMP-13表达的影响

目的探讨兔膝骨关节炎模型软骨基质金属蛋白酶(MMP)-1、-13超声波治疗前后在软骨组织中的表达及其作用机制。方法 30只新西兰大白兔随机分为正常对照组(A组)、模型组(B组)和超声波治疗组(C组),每组10只。B、C组骨关节炎造模后6周,C组实施超声波治疗,8周后取各组软骨予HE染色,免疫组化行MMP-1和MMP-13分析,观察软骨HE改变、MMP-1和MMP-13表达的变化。结果 HE染色结果采用Mankin′s评分比较,A组与B组、B组与C组比较,差异均有统计学意义(P<0.01)。软骨免疫组化MMP-1、-13测定,A组与B组、B组与C组比较,差异均有统计学意义(P<0.01)。结论超声波能有效治疗膝OA,其作用机制可能是减少软骨MMP-1、-13的含量,降低Ⅱ型胶原的降解、促进新的Ⅱ型胶原的生成,从而有利于软骨细胞外基质的合成,有利于软骨细胞的修复。

Effect of ultrasonic therapy for treating rabbit knee osteoarthritis on expression of MMP-1 and MMP-13

Objective To research the expression of MMP-1 and MMP-13 of rabbit knee cartilage cells before and after ultrasound therapy and its mechanism.Methods The normal control group(group A) contained 10 randomly selected rabbits from 30 healthy New Zealand white rabbits,not modeled,the rest 20 rabbits were randomly picked and equally divided into the model group(group B)and the ultrasonic experimental group(group C),being created osteoarthritis(OA).After 8 weeks,cartilage changes on pathological morphology and immunohistochemical of MMP-1 and MMP-13 from all groups were observed.Results In the group A,cartilage surface layer had uniform thickness,cartilage cells were aligned with uniform size,which had a little amount of MMP-1 and MMP-13.The thickness of cartilage in the group B was thinner with significantly less cartilage cells.The clustering MMP-1 and MMP-13 rates were higher.Cartilage layer of the group C was thicker than that of the group A,cartilage cells were arranged neatly,MMP-1 and MMP-13 rates were dropped.By mankin′s score,the degree of pathological lesion in the group B was higher than that of the group A and the group C(P<0.01).The expressions of MMP-1,MMP-13 were higher than those of the group A and the group C too(P<0.01).The differences were significant.Conclusion The ultrasonic treatment can effectively improve the rabbit knee OA.The mechanisms are probably reducing cartilage MMP-1 and MMP-13 levels,reducing collagen Ⅱ degradation,promoting new collagen Ⅱ formation and promoting the proteoglycan,which will help to repair cartilage cells.