HPLC测定复方蛇床子胶囊中的蛇床子素

 目的：建立复方蛇床子胶囊中蛇床子素的含量测定方法，为该新产品提供质量控制标准。方法：以体积分数为50%的乙醇为提取溶剂，超声振荡15min提取。色谱柱：Shimpack CLC-ODS(150mm×4.6mm，5μm)），保护柱：自填国产YWGC₁₈H₃₇(20mm×4.6mm，10μm)，流动相：甲醇-水-四氢呋南(70:40:5)，检测波长317nm，柱温35℃。结果：方法回收率为98.52%(n=6)，精密度为RSD＝1.44%(n=5)。结论：本实验建立的HPLC可以方便地测定复方蛇床子胶囊中的蛇床子素，可以用于复方蛇床子胶囊的质量控制。

Determination of osthole in Fufanshechuangzi capsules by HPLC

OBJECTIVE:To determine osthole in Fufangshechuangzi capsules for the quanlity control of this new preparation.METHOD:The samples were treated by ultrasonic shaking for 15min with 50% ethanol as extraction solvant. The HPLC method was penformed on Shimpack CLC-ODS column(5μm,150mm×4.6mm) with a mobile phase of methanol-water-tetrahydrofuran(70∶40∶5).The flow rate of mobile phase was 1.0ml·min^-1.A guard-column was packed with YWGC₁₈H₃₇(20mm×4.6mm,10μm).The UV detection was made at 317nm.The column tempreture was 35℃.RESULTS:The recovery of this method was 98.52%(n=6).The method reproducibility was RSD=1.44%(n=5).CONCLUSION:The method could be used to control the quality of Fufangshechuangzi capsules.