传染性单核细胞增多症患儿EBV-DNA检测及其意义

目的 探讨应用PCR技术检测EB病毒DNA(EBV-DNA)的临床应用价值.方法 应用PCR和荧光检测技术检测外周血EBV-DNA,对100例阳性病例的临床特点进行回顾性分析.结果:病例年龄1个月至12岁,中位年龄3岁,＜3岁65例,3～7岁25例,＞7岁10例;传染性单核细胞增多症(IM)组EBV-DNA的阳性率为84.0%(84/100),对照组阳性率为5.0%(2/40),差异有统计学意义(P＜0.01);IM患儿早期EBV的含量[(75.52±175.11)×103 copies/ml],明显高于恢复期EBV的含量[(0.67±2.27)×103 copies/ml](P＜0.01).结论 PCR法检测EBV-DNA时间短,准确性好,灵敏度高,在EB病毒感染相关疾病诊断中有一定价值.

Detection of epstein-barr virus DNA by fluorescent quantitative PCR in children with infectious mononucleosis

Objective To investigate the application of EBV-DNA determineation by PCR and to analyze the clinical significance in 100 EBV positive patients.Methods EBV-DNA was detected in peripheral blood by PCR and fluorescence assay.The clinical features of 100 children with EBV-DNA positive were reviewed retrospectively.Results Patients had median ages of 3 years(range,1 month to 12 years).There were 65 patients under the age of 3,25 patients between 3 and 7,and the remained older than 7 years.Among these cases,the positive rate of EBV-DNA in IM group was 84.0%(84/100)and 5.0%(2/40)in the control group,the percent of positive in the former was significant higher than the later(P＜0.01).EBV-DNA concentration of patient with IM in early stage(75.52±175.11)×103copies/ml were higher than convalescence(0.67±2.27)×103copies/ml(P＜0.01).Conclusion The EBV-DNA detection by PCR is a quick,accurate and sensitive assay in diagnosis of EBV infection diseases.