EMMPRIN及MMP-9在胃癌中的表达及其意义

目的检测细胞外基质金属蛋白酶诱导因子（extracellular matrix metalloproteinase inducer,EMMPRIN）、基质金属蛋白酶-9（matrix metalloproteinases-9,MMP-9）在胃癌中的表达,观察其与胃癌临床病理因素的关系及两者表达的相关性,探讨EMMPRIN、MMP-9在胃癌发生、发展过程中的作用。方法应用免疫组化（Elivision plus）法检测120例胃癌及癌旁正常组织中EMMPRIN及MMP-9蛋白的表达。结果癌旁组织EMMPRIN、MMP-9蛋白表达的阳性率21.7%（26/120）和19.2%（23/120）明显低于相应胃癌组织79.2%（95/120）和82.5%（99/120）,两者比较差异性显著（P〈0.01）,其表达水平与患者的性别、年龄无关（P〉0.05）,TNM分期Ⅰ、Ⅱ期胃癌组织EMMPRIN、MMP-9蛋白表达水平低于Ⅲ、Ⅳ期胃癌组织（P〈0.05）;高分化、中分化癌组织EMMPRIN、MMP-9蛋白的表达低于低、未分化癌组织（P〈0.05）;无淋巴结转移的胃癌组织EMMPRIN、MMP-9蛋白表达低于有淋巴结转移的胃癌组织（P〈0.05）,胃癌浸润程度越深表达越高（P〈0.05）;②EMMPRIN蛋白表达强度与MMP-9蛋白表达强度之间呈明显正相关（r=0.591,P〈0.001）。结论 EMMPRIN、MMP-9的表达与胃癌生物学行为密切相关,两者在胃癌浸润转移过程中可能存在协同效应。EMMPRIN、MMP-9可作为胃癌侵袭转移的判断指标。

Expression and Significance of EMMPRIN and MMP-9 in Gastric Cancer

Objective To detected the expression level of extracellular matrix metalloproteinase inducer（EMMPRIN）and matrix metalloproteinases-9（MMP-9）protein in human gastric cancer,and evaluated its relationship with the clinic pathological parameters and the role in the carcinogenesis and growth of gastric cancer.Methods Expression level of EMMPRIN and MMP-9 protein in 72 cases of gastric cancer and correspondent adjacent normal tissues was examined by immunohistochemistry envision plus method.Results ①The positive rate of EMMPRIN and MMP-9 were significantly lower in normal tissues（21.7% and 19.2%）than in gastric cancer（79.2% and 82.5%,P0.05）.No correlation were found between expression level of EMMPRIN and MMP-9 and patients sex and age（P0.05）.Expression level of EMMPRIN and MMP-9 protein decreased in stageⅠand Ⅱ of gastric cancer as compared to stage Ⅲ and Ⅳ（P0.01）.The positive rate of EMMPRIN and MMP-9 were lower in well differentiation tissues than in poor differentiation tissues（P0.01）and was higher in gastric cancer with lymph node metastasis than in those without lymph node metastasis（P0.05）.The positive rate of EMMPRIN and MMP-9 was positively correlated to the extent of invasiveness.2.A significant positive relationship was observed between the expression of EMMPRIN and MMP-9 protein in gastric cancer（r=0.591,P0.001）.Conclusion The expression level of EMMPRIN and MMP-9 protein was closely related to biological behavior of gastric cancer.EMMPRIN and MMP-9 protein in gastric cancer were associated.EMMPRIN and MMP-9 protein in the process of invasion and metastasis of gastric cancer may exist in synergy.EMMPRIN and MMP-9 protein can be used as invasion and metastasis of gastric cancer to determine the index.