| RP-HPLC 法测定不同药用部位不同采收期藤茶中蛇葡萄素的含量 |
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| 引用本文： | 王岩,周莉玲,李锐,王婴. RP-HPLC 法测定不同药用部位不同采收期藤茶中蛇葡萄素的含量[J]. 中药材, 2002, 25(1): 23-24 |
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| 作者姓名： | 王岩 周莉玲 李锐 王婴 |
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| 作者单位： | 1. 广州中医药大学,广州,510405
2. 江西制药厂,南昌,330006 |
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| 摘 要： | 目的:运用RP-HPLC法测定不同采收期藤茶根、茎和叶中蛇葡萄素的含量。方法:使用ODS色谱柱(5μm,250 mm×4.6 mm),流动相为甲醇-水-磷酸(25:75:0.1),流速1.0ml/min,检测波长289 nm。结果:藤茶叶中蛇葡萄素含量最高,茎次之,根中最低;4月至5月间采集的藤茶中蛇葡萄素含量最高。
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| 关 键 词： | 藤茶 蛇葡萄素 RP-HPLC |
| 修稿时间： | 2001-09-21 |
Determination of Ampelopsin in the Different Parts of Ampelopsis grossedentata in Different Seasons by RP-HPLC |
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| Wang Yan,Zhou Liling,Li Rui. Determination of Ampelopsin in the Different Parts of Ampelopsis grossedentata in Different Seasons by RP-HPLC[J]. Journal of Chinese medicinal materials, 2002, 25(1): 23-24 |
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| Authors: | Wang Yan Zhou Liling Li Rui |
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| Affiliation: | Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405. |
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| Abstract: | OBJECTIVE: To determine ampelopsin in the root, stem and leaf of Ampelopsis grossedentata in different seasons by RP-HPLC. METHODS: The determination was carried out with ODS column (5 microns, 250 mm x 46 mm), using methanol-water-phosphoric acid(25:75:0.1) as the mobile phase at a flow rate of 1.0 ml/min and detected at the wavelength 289 nm. RESULTS: Ampelopsin content was in the order of leaf > stem > root. Ampelopsin content in Ampelopsis grossedentata collected in April and May was the highest. |
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| Keywords: | Ampelopsis grossedentata Ampelopsin RP-HPLC |
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