| 高效液相色谱法测定四味土木香散中氧化苦参碱含量 |
| |
| 引用本文： | 马晔斐,温爱平. 高效液相色谱法测定四味土木香散中氧化苦参碱含量[J]. 中国药业, 2007, 16(21): 16-17 |
| |
| 作者姓名： | 马晔斐 温爱平 |
| |
| 作者单位： | 1. 内蒙古自治区呼和浩特市第二医院,内蒙古,呼和浩特,010030
2. 内蒙古医学院药学院,内蒙古,呼和浩特,010059 |
| |
| 摘 要： | 目的建立蒙药四味土木香散中氧化苦参碱的含量测定方法。方法采用高效液相色谱（HPLC）法，色谱柱为氨基柱，流动相为乙腈-无水乙醇-3％磷酸（80：10：10），检测波长为220nm。结果氧化苦参碱进样量在0.198-1．98μg范围内与峰面积线性关系良好，平均加样回收率为98．81％，RSD为1、49％。结论HPLC法简便快速，重现性好，灵敏度高。
|
| 关 键 词： | 高效液相色谱法 四味土木香散 氧化苦参碱 含量测定 |
| 文章编号： | 1006-4931（2007）21-0016-02 |
| 修稿时间： | 2007-02-04 |
Determination of Oxymatrine in Siweitumuxiang Powder by RP- HPLC |
| |
| Ma Yefei,Wen Aiping. Determination of Oxymatrine in Siweitumuxiang Powder by RP- HPLC[J]. China Pharmaceuticals, 2007, 16(21): 16-17 |
| |
| Authors: | Ma Yefei Wen Aiping |
| |
| Affiliation: | 1. Departrment of Pharmacy, Second Hospital of Huhehaote, Hohhot, Nei Mogol, China 010030; 2. Department of Pharmacy, Inner Mongolia Medical College, Hobbot, Nei Mogol, China 010059 |
| |
| Abstract: | Objective To establish a method for determination of oxymatrine in Siweitumuxiang Powder. Methods HPLC method was used. Hypersil APS column （200 mm×4.6 mm, 5 μm） was used. UV detector was at the wavelength of 220 nm, the mobile phase was acetonitrile-anhydrous ethanol-3% phosphoric acid （80 ： 10 ： 10） with the flow rate of 1 mL/min. The retention time of oxymatrine was 8.9 min. Results Oxymatrine showed good linear relationship in the range of 0. 198- 1.98 μg. The average recovery rate was 98.81% and RSD was 1.49%. Conclusion The method is simple, feasible and reproducible. The quality of Siweitumuxiang Powder can be controlled. |
| |
| Keywords: | HPLC Siweitumuxiang Powder oxymatrine contents determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
