| 高效液相色谱法测定多烯磷脂酰胆碱注射液中苯甲醇含量 |
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| 引用本文： | 李海英.高效液相色谱法测定多烯磷脂酰胆碱注射液中苯甲醇含量[J].中国药业,2007,16(21):21-22. |
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| 作者姓名： | 李海英 |
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| 作者单位： | 浙江省舟山市药品检验所,浙江,定海,316000 |
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| 摘 要： | 目的建立多烯磷脂酰胆碱注射液中苯甲醇的含量测定方法。方法采用高效液相色谱法，以十八炕基硅烷键合硅胶为色谱柱，甲醇-水（48：52）为流动相，检测波长254nm，流速1．0mL／min。结果苯甲醇质量浓度在0．1-2．0mg／mL范围内与峰面积呈良好的线性关系（r=0、9999），平均回收率为99．76％，RSD=0、52％（n=6）。结论该方法简便、准确，灵敏度高，适用于多烯磷脂酰胆碱注射液中苯甲醇的含量测定，
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| 关 键 词： | 多烯磷脂酰胆碱注射液 苯甲醇 高效液相色谱法 含量测定 |
| 文章编号： | 1006-4931（2007）21-0021-02 |
| 修稿时间： | 2007-08-28 |
Determination of Benzyl Alcohol in Polyene Phosphatidylcholine Injection with HPLC |
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| Li Haiying.Determination of Benzyl Alcohol in Polyene Phosphatidylcholine Injection with HPLC[J].China Pharmaceuticals,2007,16(21):21-22. |
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| Authors: | Li Haiying |
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| Institution: | Zhoushan Institute for Drug Control, Zhejiang Province, Dinghai, Zhejiang, China 316000 |
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| Abstract: | Objective To establish a method for determination of benzyl alcohol in polyene phosphatidylcholine injection. Methods HPLC method was adopted. The column was Agilent ZORBAX SB- C18 （250 mm×4. 6 mm,5 μm）, and the mobile phase was methanol- water （48： 52）. The detection wavelength was 254 nm. The flow rate was 1.0 mL/min. Results The linear range of benzyl alcohol was 0. 1 - 2.0 mg/mL, r = 0. 999 9, the average recovery rate was 99.76%, RSD = 0. 52% （ n = 6）. Conclusion This method is simple, accurate, sensitive and suitable for the determination of benzyl alcohol in polyene phosphatidylcholine injection. |
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| Keywords: | polyene phosphatidylcholine injection benzyl alcohol HPLC contents determination |
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