| 多形核粒细胞质膜结合酶活性的氧化损伤及IH－764－3的抗损伤作用 |
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| 引用本文： | 贾丽,法祥光,卢士红,刘杰文.多形核粒细胞质膜结合酶活性的氧化损伤及IH－764－3的抗损伤作用[J].中华血液学杂志,1994(5). |
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| 作者姓名： | 贾丽 法祥光 卢士红 刘杰文 |
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| 作者单位： | 中国医学科学院协和医科大学血液学研究所 |
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| 基金项目： | 国家自然科学基金，中国医学科学院青年基金 |
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| 摘 要： | 用乙酸肉豆寇咐醇酯（ＰＭＡ）刺激人多形核粒细胞（ＰＭＮ）可导致其质膜结合酶活性下降，碱性磷酸酶（ＡＬＰ）、５＇－书苷酸酶（５＇－ＮＴ）和中性内肽酶（ＮＥＰ）活性分别降至３６．６１％、５８．５３％和３０．７９％。及Ｈ＿２Ｏ＿２生成抑制试验证明，ＰＭＡ刺激后ＰＭＮ质膜结合酶活性的损伤乃活性氧物质（ＲＯＩ）所致。ＩＨ－７６４－３是自中药中提取的一种单体成分，经实验证明具有抗氧化损伤，保护ＰＭＮ质膜结合酶的作用，该抗损伤作用可能与其抑制Ｈ＿２Ｏ＿２生成有关。
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| 关 键 词： | 白细胞，质膜结合酶，氧化损伤 |
OXlDATlVE DAMAGE OF PLASMA MEMBRANE-BOUNDENZYWES OF POLYMORPHONUCLEAR LEUKOCYTEAND THE EFFECT OF lH-764-3 AGAlNST DAMAGE |
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| Jia Li,Fa Xiangguang,Lu Shihong,et al.OXlDATlVE DAMAGE OF PLASMA MEMBRANE-BOUNDENZYWES OF POLYMORPHONUCLEAR LEUKOCYTEAND THE EFFECT OF lH-764-3 AGAlNST DAMAGE[J].Chinese Journal of Hematology,1994(5). |
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| Authors: | Jia Li Fa Xiangguang Lu Shihong |
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| Abstract: | olymorphonuelear leukocytes(PMN)stimulatedwith phorbol myristate acetate (PMA) induced the de-creases of plasma membrane-bound enzyme activities ofPMN themselves.The activities of alkaline phosphatase(ALP),5'-nucleotidase (5'-NT) and neutral endopepti-dase(NEP)decreased to 36.61 %,58.53% and30. 79%,respectively.The test of and H_2O_2 pro-duction supported that the damage of the plasma mem-brane-enzymes of PMN was caused by reactive oxygenintermediates (ROI).IH-764-3, an active monomercomponent from Chinese herbs, showed the protectiveeffect to plasma membrane-bound enzymes against theoxidative damage,which was related to inhibit the gen-eration of H_2,O_2 by activated PMN themselves. |
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| Keywords: | Leukocyte Plasma membrane-boundenzyme Oxidative damage |
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