采用基因释放剂对微量及陈旧标本进行直接PCR扩增

为简化ＰＣＲ方法和减少标本用量，利用基因释放剂不提取ＤＮＡ，比较了利用基因释放剂和不用基因释放剂，对微量肝素抗凝血、微量结肠粘膜组织和含有ＰＣＲ抑制剂的ＤＮＡ标本直接进行ＰＣＲ扩增。结果，利用基因释放剂，可扩增出目的产物，不用基因释放剂 扩增出目的产物。提示利用基因释放剂直接进行ＰＣＲ，简单快还，不用提取ＤＮＡ，适于微量，含有ＰＣＲ抑制剂的ＤＮＡ及陈旧组织标本的检测。

Direct PCR amplification from samples of microliters/micrograms and long term storage by using gene releaser

In order to simplify PCR without extraction of DNA,and to detect the samples with PCR inhibitors,and to minimize the size of samples to a few microliters/micrograms,gene releaser was used. Heparinized blood samples of microliters,colonic mucosa tissues (stored in --20C over 6 months ) of micrograms and DNA samples with PCR inhibitors were checked with direct PCR with and without (as control ) gene releaser. PCR amplification product was positive with gene releaser,but negative without. Gene releaser is a very useful agent for the rapid and simplified direct PCR amplification without extraction of DNA,and suitable for detection of samples of microliters/micrograms and DNA with PCR inhibitors as well as tile long time stored tissues.