BXSB小鼠血清抗DNA抗体水平及其对淋巴细胞转化的影响

用酶联免疫吸附剂测定法（ＥＬＩＳＡ）和３Ｈ－胸腺嘧啶核苷掺入法，观察一种自发性系统性红斑狼疮（ＳＬＥ）好发小鼠（ＢＸＳＢ小鼠）在不同月龄时，血中抗ＤＮＡ抗体水平的变化，并观察该血清对正常ＬＡＣＡ小鼠淋巴细胞转化的影响。结果显示，自３月龄起，ＢＸＳＢ雄性鼠血中的抗ＤＮＡ抗体水平开始升高，４月龄上升至高峰，５月龄仍保持较高水平，各月龄ＢＸＳＢ血清与对照血清相比，Ｐ＜０．０５（ＡＮＯＶＡ）。同时，该血清对正常ＬＡＣＡ小鼠淋巴细胞转化也有抑制作用，以４月龄血清的抑制作用最大。如果将同一只小鼠血清抗ＤＮＡ抗体水平的变化与该血清对淋巴细胞转化的影响做回归和相关分析，发现两者有明显的正相关关系（，０．２３２，＜一５３，尸＜Ｏ．０５）。提示雄性＊ｘ８Ｂ小鼠可能从３月龄开始表现ＳＬＥ。发病后，血清中产生某种能够抑制淋巴细胞转化的物质，它的产生与抗ＤＮＡ抗体有平行关系。

The level of the anti-DNA antibody in the serum of BXSB mouse and the effect on lymphocyte proliferation

The level of the anti-DNA antibody in BXSB serum and the effect of BXSB serum on lymphocyte proliferation in normal mouse were observed by using enzyme-linked immunosorbent assay(ELISA) and 3H -thymidine incorpora- tion. The level of anti-DNA antibody in male BXSB serum rose from the age of 3 month and reached to the highest value at the age of 4 month. Although there were a little drop in the antibody level at the age of 5 month, it was still higher than the one of control group (P=0.032, BXSB serum vs control serum, ANOVA ). The experiment also showed that there was a dramatic negative correlation between the level of anti-DNA antibody and the effect of the same portion of BXSB serum on normal lymphocyte proliferation(r=0.232, n=53, P<0.05 ). It was concluded that the male BXSB mouse may manifest SLE from the 3rd month after birth. There generated in the SLE serum some substances which not only showed the inhibitory effect on normal lymphocyte proliferation but also were parallel, in its effect, with the level of anti-DNA antibody.