吸毒人群中HIV、TB感染者免疫细胞和细胞因子监测

目的测定吸毒人群外周血中T细胞亚群、Th1—Th2细胞因子，评价HIV、TB感染后免疫学指标的变化。方法对吸毒人群中9例HIV阳性者、5例患活动性结核病者、40例吸毒人群中健康者及40例正常人群，应用流式细胞技术（FCM）分别检测CD3^＋、CD3^＋CD4^＋．CD3^＋CD8^＋细胞百分率，计算CD3^＋CD4^＋／CD3^＋CD8^＋即Th细胞与Ts细胞的比值，采用酶联免疫吸附法（ELISA）检测Th1（IL-2、IFN-γ）/Th2（IL-4、IL-6）细胞因子浓度。结果HIV感染组、TB感染组及吸毒者健康组、正常人组各项指标间差异有统计学意义，CD3^＋CD4^＋／CD3^＋CD8^＋即Th细胞与Ts细胞的比值及Th1（IL-2、IFN-1）浓度均明显低于对照组，HIV〈TB〈吸毒人员健康者〈正常人群（P〈0．05）。而Th2（IL-4、IL-6）细胞因子浓度则明显高于正常人群。结论CD3^＋CD4^＋／CD3^＋CD8^＋即Th细胞与Ts细胞的比值及Th1（IL-2、IFN-γ）/Th2（IL-4、IL-6）细胞因子浓度的检测在评价HIV、TB感染者的免疫状况、进展和预后判断等方面具有重要作用。

Monitoring the changes of immune cells and cytokines in drug users with HIV/tuberculosis

Objective To assess the changes of immue indexes of HIV infection and TB infection by determination of T cell subsets; Th1-Th2 and sytokines in drug users . Methods Flow cytometer （FCM） was adopted to determine the levels of CD3^＋, CD3^＋CD4^＋,CD3^＋CD8^＋. The concentrations of Thl（IL-2,IFN-γ） and Th2（IL-4,IL-6） cytokines in different groups of drug users and normal persons were determined by ELISA. Results There significantly statistical differences in 9 HIV positives , 5 TB patients ,40 drug users at good health condition and 40 normal persons were observed. The ratios of CD3^＋ CD4^＋ to CD3^＋CD8^＋and concentration of Thl （IL-2,IFN-γ） were in the order of HIV group〈TB group〈normal control （P〈 0.05）,while the concentration of Th2 （IL-4,IL-6） was HIV group〉TB group〉normal control （P〈0.05）. Conclusion The ratiros of CD3^＋, CD3^＋CD4^＋ to CD3^＋CD8^＋ or the ratios of Th cell to Ts cell and the determinaion of concentrations of Thl （IL-2, IFN-γ）/Th2 （IL-4,IL-6） can be used for assessment of the immune status, development and prognosis of HIV positives and TB patients.