Pegylated TRAIL retains anti-leukemic cytotoxicity and exhibits improved signal transduction activity with respect to TRAIL |
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| Authors: | Arianna Gonelli Oriano Radillo Sara Drioli Erika Rimondi Paola Secchiero Gian Maria Bonora |
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| Affiliation: | (1) Department of Morphology and Embryology and LTTA Centre, University of Ferrara, Via Fossato di Mortara 66, 44100 Ferrara, Italy;(2) Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy;(3) Department of Chemical Science, University of Trieste, Trieste, Italy; |
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| Abstract: | To improve the pharmacokinetic profile of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) an N-terminal specific pegylation was performed to generate pegylated TRAIL (PEG-TRAIL). In in vitro experiments, we found that although PEG-TRAIL was slightly less efficient than recombinant TRAIL in promoting leukemic cell apoptosis, it showed an improved ability to promote migration of bone-marrow mesenchymal stem cells and to elicit the ERK1/2 intracellular signal transduction pathway. Overall, these data suggest that TRAIL pegylation retains, or even enhances, the biological activities of TRAIL relevant for its therapeutic applications. |
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