| 高效液相色谱法测定蒙药冬葵果中咖啡酸的含量 |
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| 引用本文： | 董玉,马强,那生桑,李霞,李树明. 高效液相色谱法测定蒙药冬葵果中咖啡酸的含量[J]. 北京中医药大学学报, 2010, 33(2): 117-119 |
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| 作者姓名： | 董玉 马强 那生桑 李霞 李树明 |
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| 作者单位： | 内蒙古医学院药学院,内蒙古,010059;内蒙古自治区科技厅 |
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| 基金项目： | 2010版《中国药典》(一部)中药标准科研资助项目(No.YD-028) |
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| 摘 要： | 目的建立冬葵果药材中咖啡酸的含量测定方法。方法RP-HPLC法:采用翡纳米科技kromasil ODS C18色谱柱(250 mm×4.6 mm,5μm),甲醇-1%冰醋酸水溶液(18∶82)等度洗脱;流速:1.0 mL/min,检测波长:330 nm;柱温:30℃。结果咖啡酸在0.027 52~0.344 0μg范围内线性关系良好。平均回收率为98.85%,RSD为2.29%(n=6),符合定量分析要求。结论结果表明,所建立的方法简便、准确,适用于冬葵果中咖啡酸的含量测定。
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| 关 键 词： | 冬葵果 咖啡酸 高效液相色谱法 含量测定 |
Quantitative determination of caffeic acid in Dongkuiguo (Fructus Malvae, Mongolian medicated herb) by HPLC |
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| DONG Yu,MA Qiang,NA Sheng-sang,LI Xia,LI Shu-ming. Quantitative determination of caffeic acid in Dongkuiguo (Fructus Malvae, Mongolian medicated herb) by HPLC[J]. Journal of Beijing University of Traditional Chinese Medicine, 2010, 33(2): 117-119 |
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| Authors: | DONG Yu MA Qiang NA Sheng-sang LI Xia LI Shu-ming |
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| Affiliation: | 1 Department of Pharmacology/a>;Inner Mongolian Medical College/a>;Inner Mongolia 010059/a>;2 Inner Mongolia Provincial Science and Technology Department |
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| Abstract: | Objective To establish a method for the quantitative determination of caffeic acid in Dongkuiguo (Fructus Malvae, Mongolian medicated herb) by RP-HPLC.Methods The procedure of RP-HPLC was performed on the chromatographic column of kromasil ODS C_(18) (250 mm×4.6 mm, 5 μm), and the mobile phase in the isocratic elution was methanol-1% acetic acid (18 to 82). The flow rate was 1.0 mL/min, the detection wavelength was 330 nm, and the temperature was at 30 ℃.Results Coffeic acid showed a good linear relationship at a range from 0.0275 2 μg to 0.344 0 μg, and the average recovery was 98.85% (RSD=2.29%, n=6).Conclusion The result of the quantitative determination shows that the method is simple and accurate, which is suitable for the quantitative determination of caffeic acid in Dongkuiguo. |
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| Keywords: | Dongkuiguo (Fructus Malvae,Mongolian medicated herb) caffeic acid HPLC quantitative determination |
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