三氧化二砷体外抑制A375黑色素瘤细胞的生长及谷胱甘肽过氧化物酶的活力

目的:了解三氧化二砷(As2O3)对体外培养的A375黑色素瘤细胞的生长及谷胱甘肽过氧化物酶活力的影响。方法:采用四甲基偶氮唑蓝(MTT)还原法检测1.5,3.0,6.0,12.0,24.0μmol/LAs2O3与A375黑色素瘤细胞作用24,48,72h后对瘤细胞的抑制作用;测定共培养24h的A375黑色素瘤细胞所含的谷胱甘肽过氧化物酶活性。结果:各浓度As2O3对A375瘤细胞的生长均有明显的抑制作用,As2O3的浓度越高,增殖抑制作用越强;各浓度As2O3与A375黑色素瘤细胞作用24h后,瘤细胞的谷胱甘肽过氧化物酶活力均有明显的降低,分别为2.23±0.81,2.87±0.70,4.08±0.81,2.63±0.84,1.40±0.41mU/mL,在As2O3浓度为6.0μmol/L时,酶活性最高,低于此浓度或高于此浓度时,酶活力呈递减的趋势。经过统计学独立样本的t检验分析,差别有统计学意义。结论:As2O3对A375瘤细胞的生长有浓度依赖性抑制作用,AsO显著降低A375瘤细胞谷胱甘肽过氧化物酶活力。

Inhibition effect of arsenic trioxide on the A375 melanoma cell growth and glutathione peroxidase activity in vitro

AIM:To understand the effect of arsenic trioxide (As2O3) on cultured A375 melanoma cell growth and glutathione peroxidase activity.METHODS:Using methyl thiazolyl tetrazolium (MTT) reduction to assay the inhibition effect of 1.5,3.0,6.0,12.0,24.0μmol/L As2O3 on A375 melanoma cells for 24,48,72 hours,respectively. To determine the glutathione peroxidase activity of the A375 melanoma cells co-cultivated with As2O3 for 24 hours. RESULTS:The growth of A375 tumor cells were inhibited by dose-depending As2O3 significantly. The higher As2O3 concentration was,the lower A375 cells proliferation was. 24 hours later,the glutathione peroxidase activity of the A375 cells was 2.23±0.81,2.87±0.70,4.08±0.81,2.63±0.84,1.40±0.41mU/mL respectively,and the 6.0μmol/L As2O3 had the highest activity. Below or above this concentration,the enzyme activity showed decreasing trend. After statistically independent samples t-tests analysis,the difference was statistically significant.CONCLUSION:As2O3 can inhibit the growth of A375 tumor cells in a dose-dependent manner and can reduce the A375 tumor cell glutathione peroxidase activity significantly.