靶向调控FASN的miRNAs在不同侵袭力骨肉瘤细胞中的表达

目的：检测可能调控脂肪酸合成酶（FASN）基因表达的miRNAs分子在不同侵袭力骨肉瘤细胞中的表达，筛选出靶向调控FASN基因的miRNAs分子，为研究骨肉瘤转移调控机制奠定基础。方法应用miRNA和microrna．org在线软件，预测可能靶向调控FASN基因（NM＿004104）表达的miRNAs分子；实时定量PCR（RT‐PCR）检测所预测到的miRNAs在骨肉瘤细胞HOS和U2‐OS中的表达；Westernblot检测HOS和U2‐OS细胞中FASN蛋白表达；Transwell侵袭实验检测HOS和U2‐OS细胞侵袭力。结果2种生物信息学预测方法预测结果均显示，FASN基因可能是miR‐195／15a／15b／16／424／497分子的靶基因；RT‐PCR结果显示，miR‐424在HOS细胞中表达水平显著高于在U2‐OS细胞中的表达水平；FASN在U2‐OS细胞中表达水平显著高于HOS细胞中的表达水平；U2‐OS细胞侵袭能力明显高于HOS细胞的侵袭力。结论miR‐424在骨肉瘤细胞中表达水平与细胞侵袭力呈负相关，miR‐424很可能通过靶向调控FASN基因影响骨肉瘤细胞侵袭转移。

Exppresion of miRNAs target regulating FASN in different invasion ability osteosarcoma cells

Objective To detect the exppresion level of miRNAs which may target fatty acid synthase(FASN) in osteosarcoma (OS) cells of diverse invasion ability ,and to select the miRNAs target regulating FASN ,acting as the basis to investigate the mech‐anism of OS metastasis .Methods The miRNA and microrna .org online software were adopted to forecast the miRNAs that might target regulate FASN(NM_004104);RT‐PCR was used to detect the expression level of the predicted miRNAs in HOS and U2‐OS cells ;the protein expression of FASN was detected by Western blot ;Transwell invasion assay was used to evaluated the invasive a‐bility of HOS and U2‐OS cells .Results Two prediction methods all showed that miR‐195/15a/15b/16/424/497 molecular might be target FASN gene;RT‐PCR result showed that the expression level of miR‐424 in HOS cells was significantly higher than that in U2‐OS cells ;the expression level of FASN was significantly higher in U2‐OS cells than that in HOS cells ;the invasive ability of U2‐OS cells is significantly higher than HOS cells .Conclusion The expression level of miR‐424 in OS cells may be negatively related to the invasive ability ,and miR‐424 likely may affect OS metastasis via targeting FASN .