神经营养素-3基因修饰骨髓间充质干细胞的明胶海绵圆柱体支架移植促进大鼠全横断脊髓损伤部分结构和功能修复的研究

目的:探讨神经营养素-3(NT-3)基因修饰骨髓间充质干细胞(MSCs)的明胶海绵圆柱体支架移植对大鼠全横断脊髓损伤部分结构和功能修复的影响。方法:选取15只SD大鼠,随机分成3组:①NT-3基因修饰MSCs联合明胶海绵圆柱体支架移植组(NT-3-MSCs组);②MSCs联合明胶海绵圆柱体支架移植组(MSCs组);③单纯明胶海绵圆柱体支架移植组(control组)。在脊髓全横断后施行上述支架移植,在一定时间点比较3组动物功能恢复情况及其脊髓损伤区的结构变化。结果:NT-3-MSCs组BBB功能评分、移植的MSCs存活数、神经丝蛋白-200(NF-200)阳性纤维计数和平均空洞面积均优于其余组别(P<0.05)。结论:NT-3基因修饰MSCs联合明胶海绵圆柱体支架移植能够促进大鼠脊髓损伤部分结构和功能的修复,为临床治疗脊髓损伤提供实验依据。

Effects of transplantation of gelatin sponge cylinder scaffold containing neurotrophin-3 NT-3 gene modified bone marrow mesenchymal stem cells on promoting the recovery of partial structure and function of rats with spinal cord complete transection injury

Abstract Objective: To explore the effects of transplantation of gelatin sponge cylinder scaffold containing neurotrophin-3(NT-3) gene modified bone marrow mesenchymal stem cells (MSCs) on the recovery of partial structure and function of rats with spinal cord complete transection injury. Method: Fifty SD rats were randomly divided into 3 groups: NT-3-MSCs group, MSCs group and control group. After complete transection of spinal cord, function recovery and morphological changes in each group were recorded and analyzed at certain time points in 8 weeks postoperation. Result: NT-3-MSCs group displayed remarkably better outcomes in BBB scores, number of survival grafted MSCs, and NF-200 positive nerve fibers as well as average cavity area than those in other groups. Conclusion: Transplantation of gelatin sponge cylinder scaffold containing NT-3 gene modified bone marrow MSCs may promote the recovery of partial structure and function of rats with spinal cord complete transection injury. It may provide a strategy for clinical spinal cord injury therapy.