经交链孢酚处理胎儿食管上皮组织中EGFr、Rb基因的扩增

Aim To study themechanism of esophageal cancer induced by alternariol (AOH). Methods DNA was extractedfrom the human fetal esophagus (HFE) treated with AOH for 24 hours,1 week and 3 weeks invitro. As controls, DNA from normal HFE, and DNA from esophageal carcinoma tissues, “normal”epithelium tissue near carcinoma region were extracted respectively. DNA samples weredigested with EcoRI enzyme and subjected to Southern blotting ,and hybridized with EGFrand Rb genes probed . Results There was amplification of EGFr gene in HFE induced by AOHin 24 hours, and the amplification continues to the third week．There were not anydeletion of Rb gene in HFE treated with AOH for 24 hours in the first week and the thirdweek． Among 10 esophageal cancer tissues, there were amplification of EGFr gene in 2cases,and complete or partial deletion of Rb gene in 2 cases and in 10 “normal”epithelium tissues near carcinoma region were found amplification of EGFr gene in 4cases,no deletion of Rb gene. Conclusion AOH could activate EGFr. The activation of EGFrgene is probably the initiated change in the canceration course of esophageal epithelium,and the inactivation of Rb anti-oncogene might be involved as late event in esophagealcarcinogenesis.

EGFr and Rb gene amplification in fetal esophageal epithelium treated by alternariol